@inbook{2baf964e18c244228d648df88c2f712c,
title = "Nontargeted Identification of d-Amino Acid-Containing Peptides Through Enzymatic Screening, Chiral Amino Acid Analysis, and LC-MS",
abstract = "d-amino acid-containing peptides (DAACPs) in animals are a class of bioactive molecules formed via the posttranslational modification of peptides consisting of all-l-amino acid residues. Amino acid residue isomerization greatly impacts the function of the resulting DAACP. However, because isomerization does not change the peptide{\textquoteright}s mass, this modification is difficult to detect by most mass spectrometry-based peptidomic approaches. Here we describe a method for the identification of DAACPs that can be used to systematically survey peptides extracted from a tissue sample in a nontargeted manner.",
keywords = "Bioactive peptides, Chirality, Peptide isomerization, Posttranslational modifications, d-amino acid-containing peptides",
author = "Samuel Okyem and Romanova, {Elena V.} and Tai, {Hua Chia} and Checco, {James W.} and Sweedler, {Jonathan V.}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3646-6_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "227--240",
booktitle = "Methods in Molecular Biology",
}