@inbook{2baf964e18c244228d648df88c2f712c,
title = "Nontargeted Identification of d-Amino Acid-Containing Peptides Through Enzymatic Screening, Chiral Amino Acid Analysis, and LC-MS",
abstract = "d-amino acid-containing peptides (DAACPs) in animals are a class of bioactive molecules formed via the posttranslational modification of peptides consisting of all-l-amino acid residues. Amino acid residue isomerization greatly impacts the function of the resulting DAACP. However, because isomerization does not change the peptide{\textquoteright}s mass, this modification is difficult to detect by most mass spectrometry-based peptidomic approaches. Here we describe a method for the identification of DAACPs that can be used to systematically survey peptides extracted from a tissue sample in a nontargeted manner.",
keywords = "Bioactive peptides, Chirality, Peptide isomerization, Posttranslational modifications, d-amino acid-containing peptides",
author = "Samuel Okyem and Romanova, {Elena V.} and Tai, {Hua Chia} and Checco, {James W.} and Sweedler, {Jonathan V.}",
note = "This work was supported by the National Institutes of Health, Award No. P30DA018310 from the National Institute on Drug Abuse (NIDA) and Award No. 2 R01NS031609 from the National Institute of Neurological Disorders and Stroke (NINDS). J.W.C. was supported in part by a Beckman Institute Postdoctoral Fellowship, funded by a Beckman Foundation gift to the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.",
year = "2024",
doi = "10.1007/978-1-0716-3646-6_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "227--240",
booktitle = "Methods in Molecular Biology",
}