Nonlinear interferometric vibrational imaging

The measurement of Raman spectra by nonlinear interferometric vibrational imaging (NIVI) to obtain the temporal anti-stokes signals was investigated. A high-resolution Raman Spectra was obtained of a molecule in biological tissues in a single pulse using broadband radiation. To show that NIVI can measure intervals of Raman spectrum in a single phase, a pulse was designed that can simulate molecules in a broad Raman spectrum The results show that the minimium discernible separation in Raman frequencies increases as the Raman frequency decreases because the anti-Stokes radiation created by lower frequency resonances overlaps more with the original spectrum.