Noninvasive diffusive optical imaging of the auditory response to birdsong in the zebra finch

James V. Lee, Edward L. Maclin, Kathy A. Low, Gabriele Gratton, Monica Fabiani, David F. Clayton

Research output: Contribution to journalArticle

Abstract

Songbirds communicate by learned vocalizations with concomitant changes in neurophysiological and genomic activities in discrete parts of the brain. Here, we tested a novel implementation of diffusive optical imaging (also known as diffuse optical imaging, DOI) for monitoring brain physiology associated with vocal signal perception. DOI noninvasively measures brain activity using red and near-infrared light delivered through optic fibers (optodes) resting on the scalp. DOI does not harm subjects, so it raises the possibility of repeatedly measuring brain activity and the effects of accumulated experience in the same subject over an entire life span, all while leaving tissue intact for further study. We developed a custom-made apparatus for interfacing optodes to the zebra finch (Taeniopygia guttata) head using 3D modeling software and rapid prototyping technology, and applied it to record responses to presentations of birdsong in isoflurane-anesthetized zebra finches. We discovered a subtle but significant difference between the hemoglobin spectra of zebra finches and mammals which has a major impact in how hemodynamic responses are interpreted in the zebra finch. Our measured responses to birdsong playback were robust, highly repeatable, and readily observed in single trials. Responses were complex in shape and closely paralleled responses described in mammals. They were localized to the caudal medial portion of the brain, consistent with response localization from prior gene expression, electrophysiological, and functional magnetic resonance imaging studies. These results define an approach for collecting neurophysiological data from songbirds that should be applicable to diverse species and adaptable for studies in awake behaving animals.

Original languageEnglish (US)
Pages (from-to)227-238
Number of pages12
JournalJournal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology
Volume199
Issue number3
DOIs
StatePublished - Jan 15 2013

Fingerprint

Finches
Taeniopygia guttata
Equidae
Optical Imaging
brain
image analysis
Brain
Songbirds
songbird
songbirds
Mammals
mammal
mammals
fiber optics
vocalization
Isoflurane
isoflurane
hemoglobin
hemodynamics
Scalp

Keywords

  • Absorption spectra
  • Anesthesia
  • Isoflurane
  • Near-infrared spectroscopy (NIRS)
  • Songbird

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Animal Science and Zoology
  • Behavioral Neuroscience

Cite this

Noninvasive diffusive optical imaging of the auditory response to birdsong in the zebra finch. / Lee, James V.; Maclin, Edward L.; Low, Kathy A.; Gratton, Gabriele; Fabiani, Monica; Clayton, David F.

In: Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology, Vol. 199, No. 3, 15.01.2013, p. 227-238.

Research output: Contribution to journalArticle

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