Nondigestible fructans alter gastrointestinal barrier function, gene expression, histomorphology, and themicrobiota profiles of diet-induced obese C57BL/6J Mice

Tzu Wen Liu, Kimberly D. Cephas, Hannah D. Holscher, Katherine R. Kerr, Heather F. Mangian, Kelly A. Tappenden, Kelly S. Swanson

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Obesity is associated with compromised intestinal barrier function and shifts in gastrointestinal microbiota that may contribute to inflammation. Fiber provides benefits, but impacts of fiber type are not understood. Objective: We aimed to determine the impact of cellulose compared with fructans on the fecal microbiota and gastrointestinal physiology in obese mice. Methods: Eighteen-wk-old male diet-induced obese C57BL/6J mice (n = 6/group; 40.5 g) were fed high-fat diets (45% kcal fat) containing 5% cellulose (control), 10% cellulose, 10% short-chain fructooligosaccharides (scFOS), or 10% inulin for 4 wk. Cecal and colon tissues were collected to assess barrier function, histomorphology, and gene expression. Fecal DNA extracts were subjected to 16S ribosomal RNA amplicon-based Illumina MiSeq sequencing to assess microbiota. Results: Body weight gain was greater (P < 0.05) in scFOS-fed than in 10% cellulose-fed mice. Both groups of fructan-fed mice had greater (P < 0.05) cecal crypt depth (scFOS: 141 μm; inulin: 145 μm) than both groups of cellulose-fedmice (5%and 10%: 109 μm). Inulin-fed mice had greater (P < 0.05) cecal transmural resistance (101 U 3 cm2) than 5% cellulose-fed controls (45 Ω 3 cm2). Inulin-fed mice had lower (P < 0.05) colonic mRNA abundance of Ocln (0.41) and Mct1 (0.35) than those fed 10% cellulose (Ocln: 1.28; Mct1: 0.90). Fructan and cellulose groups had different UniFrac distances of fecal microbiota (P < 0.05) and α diversity, which demonstrated lower (P < 0.01) species richness in fructan-fed mice. Mice fed scFOS had greater (P < 0.05) Actinobacteria (15.9%) and Verrucomicrobia (Akkermansia) (17.0%) than 5% controls (Actinobacteria: 0.07%; Akkermansia: 0.08%). Relative abundance of Akkermansia was positively correlated (r = 0.56, P < 0.01) with cecal crypt depth. Conclusions: Fructans markedly shifted gutmicrobiota and improved intestinal physiology in obesemice, but themechanisms by which they affect gut integrity and inflammation in the obese are still unknown.

Original languageEnglish (US)
Pages (from-to)949-956
Number of pages8
JournalJournal of Nutrition
Volume146
Issue number5
DOIs
StatePublished - May 1 2016

Keywords

  • Fiber
  • Gut microbiota
  • Intestinal permeability
  • Obesity
  • Tight junctions

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Nondigestible fructans alter gastrointestinal barrier function, gene expression, histomorphology, and themicrobiota profiles of diet-induced obese C57BL/6J Mice'. Together they form a unique fingerprint.

Cite this