Nondestructive volumetric imaging of tissue microstructure with benchtop x-ray phase-contrast tomography and critical point drying

Adam M. Zysk, Alfred B. Garson, Qiaofeng Xu, Eric M. Brey, Wei Zhou, Jovan G. Brankov, Miles N. Wernick, Jerome R. Kuszak, Mark A. Anastasio

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro investigation of many optically opaque biological microstructures requires 3D analysis at high resolution over a large field of view. We demonstrate a new nondestructive volumetric imaging technique that eliminates the structural and computational limitations of conventional 2D optical microscopy by combining x-ray phase-contrast tomography with critical point drying sample preparation. We experimentally demonstrate the enhancement of small features afforded by phase-contrast imaging and show the contrast improvement afforded by the drying of a hydrated specimen. We further demonstrate the biological application of this technique by imaging the microstructure of the accommodative apparatus in a primate eye using a benchtop phase-contrast tomography system.

Original languageEnglish (US)
Pages (from-to)1924-1932
Number of pages9
JournalBiomedical Optics Express
Volume3
Issue number8
DOIs
StatePublished - Aug 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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