Noncontact temperature measurement in microliter-sized volumes using fluorescent-labeled DNA oligomers

Sangmin Jeon, Jeff Turner, Steve Granick

Research output: Contribution to journalArticle

Abstract

A new method of noncontact temperature measurement in microliter-sized volumes is demonstrated, based on the temperature sensitivity of the fluorescence lifetime of rhodamine-G when it is attached to a DNA oligomer. As temperature changes, the spacing between the fluorescent dye and a designed sequence of DNA bases is modulated by conformation changes of the DNA chain, and as a result the ability of dye molecules to fluoresce is also modulated according to differential quenching by bases on the DNA. In the system that we studied, the temperature sensitivity of the fluorescence lifetime was 36-42 ps/°C depending on specific solution conditions. Although this strategy of temperature measurement is demonstrated using a specific sequence of DNA, it can also be generalized to a dye attached to any other intrinsic quencher of fluorescence whose conformation changes with temperature.

Original languageEnglish (US)
Pages (from-to)9908-9909
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number33
DOIs
StatePublished - Aug 20 2003

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Noncontact temperature measurement in microliter-sized volumes using fluorescent-labeled DNA oligomers'. Together they form a unique fingerprint.

  • Cite this