Abstract
(Graph Presented) We have synthesized a new SNAPFL analogue (1) that exhibits a large Stokes shift (78 nm) (abs. 542 nm, em. 620 nm) and a good quantum yield. Because of the large overlap between the emission spectrum of 1 and the absorption spectrum of Cy5, 1 functions well as a fluorescence donor to Cy5 and has been used in FRET-based experiments using estrogen receptor site-specifically labeled with Cy5 and a receptor ligand conjugated to SNAPFL.
Original language | English (US) |
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Pages (from-to) | 4931-4934 |
Number of pages | 4 |
Journal | Organic Letters |
Volume | 10 |
Issue number | 21 |
DOIs | |
State | Published - 2008 |
ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry