Nonblinking and long-lasting single-molecule fluorescence imaging

Ivan Rasnik, Sean A. McKinney, Taekjip Ha

Research output: Contribution to journalArticlepeer-review

Abstract

Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.

Original languageEnglish (US)
Pages (from-to)891-893
Number of pages3
JournalNature Methods
Volume3
Issue number11
DOIs
StatePublished - Nov 2006

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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