Non-targeted identification of d-amino acid-containing peptides through enzymatic screening, chiral amino acid analysis, and LC-MS

Hua Chia Tai, James W. Checco, Jonathan V. Sweedler

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

d-Amino acid-containing peptides (DAACPs) in animals are a class of bioactive molecules formed via the posttranslational modification of peptides consisting of all-l-amino acid residues. Amino acid residue isomerization greatly impacts the function of the resulting DAACP. However, because isomerization does not change the peptide’s mass, this modification is difficult to detect by most mass spectrometry-based peptidomic approaches. Here we describe a method for the identification of DAACPs that can be used to systematically survey peptides extracted from a tissue sample in a non-targeted manner.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-118
Number of pages12
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1719
ISSN (Print)1064-3745

Keywords

  • Bioactive peptides
  • Chirality
  • Peptide isomerization
  • Posttranslational modifications
  • d-Amino acid-containing peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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