Non-fluorescent quantification of single mRNA with transient absorption microscopy

Research output: Contribution to journalArticle

Abstract

Single molecule detection is confounded by the background signals from the biological environment, such as autofluorescence, Rayleigh scattering, or turbidity in cells and tissues. In this article, we report on the utilization of gold nanoparticles (AuNPs) as an orthogonal probe for non-fluorescence detection of single molecules with a transient absorption microscopy (TAM). The developed system and concepts were validated by quantitative evaluation of human epidermal receptor 2 (Her2) mRNA in cancer cells and tissues at single copy sensitivity. Results from TAM suggest that the average number of Her2 copies in SK-BR-3 and MCF-7 breast cancer cells is 203.19 ± 80.48, and 11.29 ± 4.47, respectively. Furthermore, TAM offers excellent signal-to-noise ratio in detecting mRNA in clinical tissues, indicating a significantly higher expression of Her2 genes in breast cancer tissues than that of normal tissues. Our single cell quantification TAM strategy was validated with a fluorescence in situ hybridization approach. Our demonstration shows that TAM has the potential to provide a new dimension in biomarker quantification at single molecule sensitivity in turbid biological environments providing a strong basis for clinical monitoring.

Original languageEnglish (US)
Pages (from-to)19242-19248
Number of pages7
JournalNanoscale
Volume8
Issue number46
DOIs
StatePublished - Dec 14 2016
Externally publishedYes

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Microscopic examination
Tissue
Messenger RNA
Molecules
Cells
Rayleigh scattering
Biomarkers
Turbidity
Gold
Signal to noise ratio
Demonstrations
Genes
Fluorescence
Nanoparticles
Monitoring

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Non-fluorescent quantification of single mRNA with transient absorption microscopy. / Liu, Jing; Irudayaraj, Joseph Maria Kumar.

In: Nanoscale, Vol. 8, No. 46, 14.12.2016, p. 19242-19248.

Research output: Contribution to journalArticle

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