Abstract
Estrogen receptor (ER)-associated cofactors and cooperating transcription factors are one of the primary components determining transcriptional activity of estrogen target genes and may constitute potential therapeutic targets. Recent mapping of ER-binding sites on a genome-wide scale has provided insight into novel cooperating factors based on the enrichment of transcription factor motifs within the ER-binding sites. We have used the ER-binding sites in combination with sequence conservation to identify the statistical enrichment of Nkxand LEF motifs. We find that Nkx3-1 and LEF-1 bind to several ER cis-regulatory elements in vivo, but they both function as transcriptional repressors of estrogen signaling. We show that Nkx3-1 and LEF-1 can inhibit ER binding to chromatin, suggesting competition for common chromatin-binding regions. These data provide insight into the role of Nkx3-1 and LEF-1 as potential regulators of the hormone response inbreast cancer.
Original language | English (US) |
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Pages (from-to) | 7380-7385 |
Number of pages | 6 |
Journal | Cancer Research |
Volume | 68 |
Issue number | 18 |
DOIs | |
State | Published - Sep 15 2008 |
Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research