Nitric oxide synthase inhibitor blocks light-induced phase shifts of the circadian activity rhythm, but not c-fos expression in the suprachiasmatic nucleus of the Syrian hamster

E. Todd Weber, Robert L. Gannon, Anna Marie Michel, Martha U. Gillette, Michael A. Rea

Research output: Contribution to journalArticle

Abstract

Circadian rhythms in mammals are entrained to the environmental light cycle by daily adjustments in the phase of the circadian pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. Brief exposure of hamsters maintained under constant darkness to ambient light during subjective nighttime produces both phase shifts of the circadian activity rhythm and characteristic patterns of c-fos protein (Fos) immunoreactivity in the SCN. In this study, we demonstrate that light-induced phase shifts of the circadian activity rhythm are blocked by intracerebroventricular (i.c.v.) injection of the competitive nitric oxide synthase (NOS) inhibitor,N-nitro-l-arginine methyl ester (l-NAME), but not by the inactive isomer,d-NAME. The effects ofl-NAME are reversible and dose-related, and are countered by co-injection of arginine, the natural substrate for NOS. While effects on behavioral rhythms are pronounced, similar treatment does not alter the pattern of light-induced Fos immunoreactivity in the SCN. These results suggest that nitric oxide is a component of the signal transduction pathway that communicates photic information to the SCN circadian pacemaker, and that nitric oxide production is either independent of, or downstream from, pathways involved in induction of c-fos expression.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalBrain Research
Volume692
Issue number1-2
DOIs
StatePublished - Sep 18 1995

Fingerprint

Suprachiasmatic Nucleus
Mesocricetus
Circadian Rhythm
Nitric Oxide Synthase
Light
Nitric Oxide
Proto-Oncogene Proteins c-fos
Injections
Darkness
Photoperiod
Cricetinae
Hypothalamus
Arginine
Mammals
Signal Transduction
Therapeutics

Keywords

  • Circadian rhythm
  • Fos
  • Nitric oxide
  • Photic entrainment
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Nitric oxide synthase inhibitor blocks light-induced phase shifts of the circadian activity rhythm, but not c-fos expression in the suprachiasmatic nucleus of the Syrian hamster. / Todd Weber, E.; Gannon, Robert L.; Michel, Anna Marie; Gillette, Martha U.; Rea, Michael A.

In: Brain Research, Vol. 692, No. 1-2, 18.09.1995, p. 137-142.

Research output: Contribution to journalArticle

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