TY - JOUR
T1 - Nitric oxide synthase imunolabeling in the molluscan CNS and peripheral tissues
AU - Hurst, W. J.
AU - Moroz, L. L.
AU - Gillette, M. U.
AU - Gillette, R.
N1 - Funding Information:
This research was supported by NIH research grants NS33230 to M.U.G., MH59339 to R.G., and, in part, by INTAS (#93-3504), and International Research Scholars Program (#75195-540101; HHMI) and NIH 39103-01 grants to L.L.M. We thank Brend Mayer (University of Graz) for anti-nNOS and baculovirus-expressed nNOS.
PY - 1999/8/27
Y1 - 1999/8/27
N2 - NOS immunoreactivity was assayed in CNS and peripheral tissues of the sea slugs Pleurobranchaea californica, Tritonia diomedea and Aplysia californica using different antisera against mammalian nitric oxide synthase in Western blots. Polyclonal anti-nNOS labeled at 250, 185, 170, 155, 100, 75, and 65 kD in extracts of Pleurobranchaea CNS, salivary gland and esophagus but not of gills or muscle. The labeling pattern for Tritonia in bands at 250, 200, 120/110, 100, 69, 65, and 60 kD differed somewhat. Anti-nNOS labeling in Aplysia was markedly different, with bands labeled only at 69 and 60 kD in CNS extracts, and at 200, 190, 69 and 60 kD in salivary and esophagus extracts. The wide variation in NOS immunoreactivity is consistent with species differences in tissue localization and biochemical properties of molluscan NOS isoforms.
AB - NOS immunoreactivity was assayed in CNS and peripheral tissues of the sea slugs Pleurobranchaea californica, Tritonia diomedea and Aplysia californica using different antisera against mammalian nitric oxide synthase in Western blots. Polyclonal anti-nNOS labeled at 250, 185, 170, 155, 100, 75, and 65 kD in extracts of Pleurobranchaea CNS, salivary gland and esophagus but not of gills or muscle. The labeling pattern for Tritonia in bands at 250, 200, 120/110, 100, 69, 65, and 60 kD differed somewhat. Anti-nNOS labeling in Aplysia was markedly different, with bands labeled only at 69 and 60 kD in CNS extracts, and at 200, 190, 69 and 60 kD in salivary and esophagus extracts. The wide variation in NOS immunoreactivity is consistent with species differences in tissue localization and biochemical properties of molluscan NOS isoforms.
KW - Aplysia
KW - Endothelial NOS
KW - Gastropoda
KW - Inducible NOS
KW - Neuronal NOS
KW - Pleurobranchaea
KW - Tritonia
UR - http://www.scopus.com/inward/record.url?scp=0033610014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033610014&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.1247
DO - 10.1006/bbrc.1999.1247
M3 - Article
C2 - 10462511
AN - SCOPUS:0033610014
SN - 0006-291X
VL - 262
SP - 545
EP - 548
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -