New wine from old barrels

Research output: Contribution to journalShort survey

Abstract

Phosphoribosylanthranilate isomerase (PRAI) and indole-3-glycerol phosphate synthase (IGPS) are distantly related α/β-barrel enzymes that catalyze successive steps in tryptophan biosynthesis. A 'new' PRAI was evolved from an 'old' IGPS by random mutagenesis followed by DNA shuffling and in vivo selection.

Original languageEnglish (US)
Pages (from-to)171-173
Number of pages3
JournalNature Structural Biology
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2000

Fingerprint

Indole-3-Glycerol-Phosphate Synthase
Wine
DNA Shuffling
Mutagenesis
Biosynthesis
Tryptophan
DNA
Enzymes
phosphoribosylanthranilate isomerase

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

New wine from old barrels. / Gerlt, John Alan.

In: Nature Structural Biology, Vol. 7, No. 3, 01.03.2000, p. 171-173.

Research output: Contribution to journalShort survey

Gerlt, John Alan. / New wine from old barrels. In: Nature Structural Biology. 2000 ; Vol. 7, No. 3. pp. 171-173.
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