New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Yunzi Luo, Behnam Enghiad, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.

Original languageEnglish (US)
Pages (from-to)174-182
Number of pages9
JournalNatural Product Reports
Volume33
Issue number2
DOIs
StatePublished - Feb 2016

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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