New reagent for discrimination of single- and double-stranded regions in DNA

A. V. Mazin, A. V. Kuzminov, G. L. Dianov, R. I. Salganik

Research output: Contribution to journalArticlepeer-review


It was found that DNA alkylation at the N-7 guanine with the bulky alkylating reagent, N,N,N'-tri-(β-chloroethyl)-N-(p-formylpheny)propylene diamine-1,3 (TFP) is much diminished when DNA is double-stranded. We report here an application of this reaction for probing the hairpin structure in the palindrome-containing single-stranded (ss) DNA fragment of 377 bases prepared from the EcoRI-BamHI fragment of plasmid pBR322. 5'-Labeled ss fragment was modified with TFP and cleaved by piperidine hydrolysis at the alkylated guanine residues according to the Maxam-Gilbert procedure. Guanines in the hairpin formed by palindrome of 9 bp were protected from TFP action, while dimethyl sulfate modified all guanines.

Original languageEnglish (US)
Pages (from-to)244-246
Number of pages3
JournalFEBS Letters
Issue number2
StatePublished - Dec 4 1989
Externally publishedYes


  • Bulky alkylating reagent
  • Guanine alkylation, N-7
  • Structure probing, hairpin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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