New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein

Sang Hyuk Han, Jong Seo Park, Jae Yeong Han, Jun Su Gong, Chan Hwan Park, Jung Kyu Kim, Eun Young Seo, Leslie L. Domier, John Hammond, Hyoun Sub Lim

Research output: Contribution to journalArticle

Abstract

Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76. In addition, we generated infectious clones of S-47 and J-76, and T7 promoter driven transcripts of each inoculated to Nicotiana benthamiana produced very severe symptoms, whereas only mild symptoms developed in Capsicum annuum. Gene silencing suppressor function of 126 kDa and cytoskeleton-connected plasmodesmata localization of movement protein of S-47 and J-76 showed no difference between isolates, whereas 126 kDa of J-76 clearly formed intracellular aggregates not observed with S-47 126 kDa protein. Differences between these isolates in 126/183 kDa-related functions including subcellular localization suggest that differential interactions with host proteins may affect symptom development in C. annuum.

Original languageEnglish (US)
Pages (from-to)434-445
Number of pages12
JournalVirus Genes
Volume53
Issue number3
DOIs
StatePublished - Jun 1 2017

Keywords

  • Full-length infectious cDNA clone
  • In vitro transcription
  • Nationwide survey
  • Seed transmission
  • T7 promoter

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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    Han, S. H., Park, J. S., Han, J. Y., Gong, J. S., Park, C. H., Kim, J. K., Seo, E. Y., Domier, L. L., Hammond, J., & Lim, H. S. (2017). New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein. Virus Genes, 53(3), 434-445. https://doi.org/10.1007/s11262-017-1432-4