New insights into the evolution and functional divergence of the SWEET family in Saccharum based on comparative genomics

Weichang Hu, Xiuting Hua, Qing Zhang, Jianping Wang, Qiaochu Shen, Xingtan Zhang, Kai Wang, Qingyi Yu, Yann Rong Lin, Ray R Ming, Jisen Zhang

Research output: Contribution to journalArticle

Abstract

Background: The SWEET (Sugars Will Eventually be Exported Transporters) gene family is a recently identified group of sugar transporters that play an indispensable role in sugar efflux, phloem loading, plant-pathogen interaction, nectar secretion, and reproductive tissue development. However, little information on Saccharum SWEET is available for this crop with a complex genetic background. Results: In this study, 22SWEET genes were identified from Saccharum spontaneum Bacterial Artificial Chromosome libraries sequences. Phylogenetic analyses of SWEETs from 11 representative plant species showed that gene expansions of the SWEET family were mainly caused by the recent gene duplication in dicot plants, while these gene expansions were attributed to the ancient whole genome duplication (WGD) in monocot plant species. Gene expression profiles were obtained from RNA-seq analysis. SWEET1a and SWEET2s had higher expression levels in the transitional zone and maturing zone than in the other analyzed zones. SWEET1b was mainly expressed in the leaf tissues and the mature zone of the leaf of both S. spontaneum and S. officinarum, and displayed a peak in the morning and was undetectable in both sclerenchyma and parenchyma cells from the mature stalks of S. officinarum. SsSWEET4a\4b had higher expression levels than SWEET4c and were mainly expressed in the stems of seedlings and mature plants. SWEET13s are recently duplicated genes, and the expression of SWEET13s dramatically increased from the maturing to mature zones. SWEET16b's expression was not detected in S. officinarum, but displayed a rhythmic diurnal expression pattern. Conclusions: Our study revealed the gene evolutionary history of SWEETs in Saccharum and SWEET1b was found to be a sucrose starvation-induced gene involved in the sugar transportation in the high photosynthetic zones. SWEET13c was identified as the key player in the efflux of sugar transportation in mature photosynthetic tissues. SWEET4a\4b were found to be mainly involved in sugar transportation in the stalk. SWEET1a\2a\4a\4b\13a\16b were suggested to be the genes contributing to the differences in sugar contents between S. spontaneum and S. officinarum. Our results are valuable for further functional analysis of SWEET genes and utilization of the SWEET genes for genetic improvement of Saccharum for biofuel production.

Original languageEnglish (US)
Article number270
JournalBMC Plant Biology
Volume18
Issue number1
DOIs
StatePublished - Nov 7 2018

Fingerprint

Saccharum
transporters
sugars
genomics
Saccharum spontaneum
genes
phloem loading
sclerenchyma
nectar secretion
bacterial artificial chromosomes
DNA libraries
gene duplication
Magnoliopsida
Liliopsida
mature plants
biofuels
plant pathogens
sugar content
genetic background
parenchyma (plant tissue)

Keywords

  • Gene evolution
  • Gene expression
  • SWEET
  • Saccharum officinarum
  • Saccharum spontaneum
  • Sugar transport

ASJC Scopus subject areas

  • Plant Science

Cite this

New insights into the evolution and functional divergence of the SWEET family in Saccharum based on comparative genomics. / Hu, Weichang; Hua, Xiuting; Zhang, Qing; Wang, Jianping; Shen, Qiaochu; Zhang, Xingtan; Wang, Kai; Yu, Qingyi; Lin, Yann Rong; Ming, Ray R; Zhang, Jisen.

In: BMC Plant Biology, Vol. 18, No. 1, 270, 07.11.2018.

Research output: Contribution to journalArticle

Hu, Weichang ; Hua, Xiuting ; Zhang, Qing ; Wang, Jianping ; Shen, Qiaochu ; Zhang, Xingtan ; Wang, Kai ; Yu, Qingyi ; Lin, Yann Rong ; Ming, Ray R ; Zhang, Jisen. / New insights into the evolution and functional divergence of the SWEET family in Saccharum based on comparative genomics. In: BMC Plant Biology. 2018 ; Vol. 18, No. 1.
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AU - Hu, Weichang

AU - Hua, Xiuting

AU - Zhang, Qing

AU - Wang, Jianping

AU - Shen, Qiaochu

AU - Zhang, Xingtan

AU - Wang, Kai

AU - Yu, Qingyi

AU - Lin, Yann Rong

AU - Ming, Ray R

AU - Zhang, Jisen

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N2 - Background: The SWEET (Sugars Will Eventually be Exported Transporters) gene family is a recently identified group of sugar transporters that play an indispensable role in sugar efflux, phloem loading, plant-pathogen interaction, nectar secretion, and reproductive tissue development. However, little information on Saccharum SWEET is available for this crop with a complex genetic background. Results: In this study, 22SWEET genes were identified from Saccharum spontaneum Bacterial Artificial Chromosome libraries sequences. Phylogenetic analyses of SWEETs from 11 representative plant species showed that gene expansions of the SWEET family were mainly caused by the recent gene duplication in dicot plants, while these gene expansions were attributed to the ancient whole genome duplication (WGD) in monocot plant species. Gene expression profiles were obtained from RNA-seq analysis. SWEET1a and SWEET2s had higher expression levels in the transitional zone and maturing zone than in the other analyzed zones. SWEET1b was mainly expressed in the leaf tissues and the mature zone of the leaf of both S. spontaneum and S. officinarum, and displayed a peak in the morning and was undetectable in both sclerenchyma and parenchyma cells from the mature stalks of S. officinarum. SsSWEET4a\4b had higher expression levels than SWEET4c and were mainly expressed in the stems of seedlings and mature plants. SWEET13s are recently duplicated genes, and the expression of SWEET13s dramatically increased from the maturing to mature zones. SWEET16b's expression was not detected in S. officinarum, but displayed a rhythmic diurnal expression pattern. Conclusions: Our study revealed the gene evolutionary history of SWEETs in Saccharum and SWEET1b was found to be a sucrose starvation-induced gene involved in the sugar transportation in the high photosynthetic zones. SWEET13c was identified as the key player in the efflux of sugar transportation in mature photosynthetic tissues. SWEET4a\4b were found to be mainly involved in sugar transportation in the stalk. SWEET1a\2a\4a\4b\13a\16b were suggested to be the genes contributing to the differences in sugar contents between S. spontaneum and S. officinarum. Our results are valuable for further functional analysis of SWEET genes and utilization of the SWEET genes for genetic improvement of Saccharum for biofuel production.

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KW - Gene evolution

KW - Gene expression

KW - SWEET

KW - Saccharum officinarum

KW - Saccharum spontaneum

KW - Sugar transport

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