New directions of activity-based sensing forin vivoNIR imaging

In vivoimaging is a powerful approach to study biological processes. Beyond cellular methods,in vivostudies allow for biological stimuli (small molecules or proteins) to be studied in their native environment. This has the potential to aid in the discovery of new biology and guide the development of diagnostics and therapies for diseases. To ensure selectivity and an observable readout, the probe development field is shifting towards activity-based sensing (ABS) approaches and near-infrared (NIR) imaging modalities. This perspective will highlight recentin vivoABS applications that utilize NIR imaging platforms.