@article{a5f7bb3e0e8446f4a4c0deff4ab4282f,
title = "Neutralizing Antibodies to SARS-CoV-2 Selected from a Human Antibody Library Constructed Decades Ago",
abstract = "Combinatorial antibody libraries not only effectively reduce antibody discovery to a numbers game, but enable documentation of the history of antibody responses in an individual. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has prompted a wider application of this technology to meet the public health challenge of pandemic threats in the modern era. Herein, a combinatorial human antibody library constructed 20 years before the coronavirus disease 2019 (COVID-19) pandemic is used to discover three highly potent antibodies that selectively bind SARS-CoV-2 spike protein and neutralize authentic SARS-CoV-2 virus. Compared to neutralizing antibodies from COVID-19 patients with generally low somatic hypermutation (SHM), these three antibodies contain over 13–22 SHMs, many of which are involved in specific interactions in their crystal structures with SARS-CoV-2 spike receptor binding domain. The identification of these somatically mutated antibodies in a pre-pandemic library raises intriguing questions about the origin and evolution of these antibodies with respect to their reactivity with SARS-CoV-2.",
author = "Min Qiang and Peixiang Ma and Yu Li and Hejun Liu and Adam Harding and Chenyu Min and Fulian Wang and Lili Liu and Meng Yuan and Qun Ji and Pingdong Tao and Xiaojie Shi and Zhean Li and Teng Li and Xian Wang and Yu Zhang and Wu, {Nicholas C.} and Lee, {Chang Chun D.} and Xueyong Zhu and Javier Gilbert-Jaramillo and Chuyue Zhang and Abhishek Saxena and Xingxu Huang and Hou Wang and William James and Dwek, {Raymond A.} and Wilson, {Ian A.} and Guang Yang and Lerner, {Richard A.}",
note = "Funding Information: The authors thank Dr. Lichun Jiang, Dr. Wei Wang, and Zhangyue Song from the Biomedical Big Data platform of the Shanghai Institute for Advanced Immunochemical Studies (SIAIS) of ShanghaiTech University for sequencing and data analysis, Pengwei Zhang, Dr. Lishuang Zhang, and Juan Kong from the High‐Throughput Screening Platform of SIAIS for technical support in cell sorting and phage panning, and Jiakang Chen from the Analytical Chemistry Platform of SIAIS for technical support in SEC‐HPLC. Work at ShanghaiTech University was supported by Emergency Key Program of Guangzhou Laboratory (Grants number EKPG21‐18), National Natural Science Foundation of China (Grants number 31500632), the China Evergrande Group (Grants number 2020GIRHHMS05), and Shanghai Local Grant (Grants number ZJ2020‐ZD‐004). JPB Foundation supported the work in the Lerner Lab, and The Bill and Melinda Gates Foundation OPP1170236 and INV‐004923 provided support to the Wilson lab. X‐ray data collection used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE‐AC02‐06CH11357. Extraordinary facility operations were supported in part by the DOE Office of Science through the National Virtual Biotechnology Laboratory, a consortium of DOE national laboratories focused on the response to COVID‐19, with funding provided by the Coronavirus CARES Act. Funding Information: The authors thank Dr. Lichun Jiang, Dr. Wei Wang, and Zhangyue Song from the Biomedical Big Data platform of the Shanghai Institute for Advanced Immunochemical Studies (SIAIS) of ShanghaiTech University for sequencing and data analysis, Pengwei Zhang, Dr. Lishuang Zhang, and Juan Kong from the High-Throughput Screening Platform of SIAIS for technical support in cell sorting and phage panning, and Jiakang Chen from the Analytical Chemistry Platform of SIAIS for technical support in SEC-HPLC. Work at ShanghaiTech University was supported by Emergency Key Program of Guangzhou Laboratory (Grants number EKPG21-18), National Natural Science Foundation of China (Grants number 31500632), the China Evergrande Group (Grants number 2020GIRHHMS05), and Shanghai Local Grant (Grants number ZJ2020-ZD-004). JPB Foundation supported the work in the Lerner Lab, and The Bill and Melinda Gates Foundation OPP1170236 and INV-004923 provided support to the Wilson lab. X-ray data collection used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Extraordinary facility operations were supported in part by the DOE Office of Science through the National Virtual Biotechnology Laboratory, a consortium of DOE national laboratories focused on the response to COVID-19, with funding provided by the Coronavirus CARES Act. Publisher Copyright: {\textcopyright} 2021 The Authors. Advanced Science published by Wiley-VCH GmbH",
year = "2022",
month = jan,
day = "5",
doi = "10.1002/advs.202102181",
language = "English (US)",
volume = "9",
journal = "Advanced Science",
issn = "2198-3844",
publisher = "Wiley-VCH",
number = "1",
}