Neutralization of multiple Staphylococcal superantigens by a single-chain protein consisting of affinity-matured, variable domain repeats

Xi Yang, Rebecca A. Buonpane, Beenu Moza, A. K.M.Nur Ur Rahman, Ningyan Wang, Patrick M. Schlievert, John K. McCormick, Eric J. Sundberg, David M Kranz

Research output: Contribution to journalArticle

Abstract

Staphylococcus aureus secretes various toxins that act as superantigens by stimulating a large fraction of the host's T cells. Toxin binding to variable domains of T cell receptor β chains (Vβ) leads to massive release of inflammatory molecules and potentially to toxic shock syndrome (TSS). Previously, we generated soluble forms of different Vβ domains with a high affinity for binding superantigens. However, a broader spectrum antagonist is required for the neutralization of multiple toxins. In the present study, we expressed Vβ domains in tandem as a single-chain protein and neutralized the clinically important superantigens staphylococcal enterotoxin B and TSS toxin-1 with a single agent.

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalJournal of Infectious Diseases
Volume198
Issue number3
DOIs
StatePublished - Aug 1 2008

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Superantigens
Proteins
Septic Shock
T-Cell Antigen Receptor
Staphylococcus aureus
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Neutralization of multiple Staphylococcal superantigens by a single-chain protein consisting of affinity-matured, variable domain repeats. / Yang, Xi; Buonpane, Rebecca A.; Moza, Beenu; Rahman, A. K.M.Nur Ur; Wang, Ningyan; Schlievert, Patrick M.; McCormick, John K.; Sundberg, Eric J.; Kranz, David M.

In: Journal of Infectious Diseases, Vol. 198, No. 3, 01.08.2008, p. 344-348.

Research output: Contribution to journalArticle

Yang, X, Buonpane, RA, Moza, B, Rahman, AKMNU, Wang, N, Schlievert, PM, McCormick, JK, Sundberg, EJ & Kranz, DM 2008, 'Neutralization of multiple Staphylococcal superantigens by a single-chain protein consisting of affinity-matured, variable domain repeats', Journal of Infectious Diseases, vol. 198, no. 3, pp. 344-348. https://doi.org/10.1086/589776
Yang, Xi ; Buonpane, Rebecca A. ; Moza, Beenu ; Rahman, A. K.M.Nur Ur ; Wang, Ningyan ; Schlievert, Patrick M. ; McCormick, John K. ; Sundberg, Eric J. ; Kranz, David M. / Neutralization of multiple Staphylococcal superantigens by a single-chain protein consisting of affinity-matured, variable domain repeats. In: Journal of Infectious Diseases. 2008 ; Vol. 198, No. 3. pp. 344-348.
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