Abstract

Neuropeptidomics refers to a global characterization approach for the investigation of neuropeptides, often under specific physiological conditions. Neuropeptides comprise a complex set of signaling molecules that are involved in regulatory functions and behavioral control in the nervous system. Neuropeptidomics is inherently challenging because neuropeptides are spatially, temporally, and chemically heterogeneous, making them difficult to predict in silico from genomic information. Mature neuropeptides are produced from intricate enzymatic processing of precursor proteins/prohormones via a range of posttranslational modifications, resulting in multiple final peptide products from each prohormone gene. Although there are several methods for targeted peptide studies, mass spectrometry (MS), with its qualitative and quantitative capabilities, is ideally suited to the task. MS provides fast, sensitive, accurate, and high-throughput peptidomic analysis of neuropeptides without requiring prior knowledge of the peptide sequences. Aided by liquid chromatography (LC) separations and bioinformatics, MS is quickly becoming a leading technique in neuropeptidomics. This chapter describes several LC-MS analytical methods to identify, characterize, and quantify neuropeptides while emphasizing the sample preparation steps so integral to experimental success.

Original languageEnglish (US)
Title of host publicationNeuropeptides
Subtitle of host publicationMethods and Protocols
EditorsAdalberto Merighi
Pages223-236
Number of pages14
DOIs
StatePublished - Oct 24 2011

Publication series

NameMethods in Molecular Biology
Volume789
ISSN (Print)1064-3745

Fingerprint

Neuropeptides
Mass Spectrometry
Liquid Chromatography
Peptides
Protein Precursors
Post Translational Protein Processing
Computational Biology
Computer Simulation
Nervous System
Genes

Keywords

  • Liquid chromatography
  • Mass spectrometry
  • Peptide identification
  • Quantitation
  • Sample preparation
  • Stable isotope labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yin, P., Hou, X., Romanova, E. V., & Sweedler, J. V. (2011). Neuropeptidomics: Mass spectrometry-based qualitative and quantitative analysis. In A. Merighi (Ed.), Neuropeptides: Methods and Protocols (pp. 223-236). (Methods in Molecular Biology; Vol. 789). https://doi.org/10.1007/978-1-61779-310-3_14

Neuropeptidomics : Mass spectrometry-based qualitative and quantitative analysis. / Yin, Ping; Hou, Xiaowen; Romanova, Elena Vikentievna; Sweedler, Jonathan V.

Neuropeptides: Methods and Protocols. ed. / Adalberto Merighi. 2011. p. 223-236 (Methods in Molecular Biology; Vol. 789).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yin, P, Hou, X, Romanova, EV & Sweedler, JV 2011, Neuropeptidomics: Mass spectrometry-based qualitative and quantitative analysis. in A Merighi (ed.), Neuropeptides: Methods and Protocols. Methods in Molecular Biology, vol. 789, pp. 223-236. https://doi.org/10.1007/978-1-61779-310-3_14
Yin P, Hou X, Romanova EV, Sweedler JV. Neuropeptidomics: Mass spectrometry-based qualitative and quantitative analysis. In Merighi A, editor, Neuropeptides: Methods and Protocols. 2011. p. 223-236. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-310-3_14
Yin, Ping ; Hou, Xiaowen ; Romanova, Elena Vikentievna ; Sweedler, Jonathan V. / Neuropeptidomics : Mass spectrometry-based qualitative and quantitative analysis. Neuropeptides: Methods and Protocols. editor / Adalberto Merighi. 2011. pp. 223-236 (Methods in Molecular Biology).
@inbook{f1a6da68725447bd8821f1872adcf907,
title = "Neuropeptidomics: Mass spectrometry-based qualitative and quantitative analysis",
abstract = "Neuropeptidomics refers to a global characterization approach for the investigation of neuropeptides, often under specific physiological conditions. Neuropeptides comprise a complex set of signaling molecules that are involved in regulatory functions and behavioral control in the nervous system. Neuropeptidomics is inherently challenging because neuropeptides are spatially, temporally, and chemically heterogeneous, making them difficult to predict in silico from genomic information. Mature neuropeptides are produced from intricate enzymatic processing of precursor proteins/prohormones via a range of posttranslational modifications, resulting in multiple final peptide products from each prohormone gene. Although there are several methods for targeted peptide studies, mass spectrometry (MS), with its qualitative and quantitative capabilities, is ideally suited to the task. MS provides fast, sensitive, accurate, and high-throughput peptidomic analysis of neuropeptides without requiring prior knowledge of the peptide sequences. Aided by liquid chromatography (LC) separations and bioinformatics, MS is quickly becoming a leading technique in neuropeptidomics. This chapter describes several LC-MS analytical methods to identify, characterize, and quantify neuropeptides while emphasizing the sample preparation steps so integral to experimental success.",
keywords = "Liquid chromatography, Mass spectrometry, Peptide identification, Quantitation, Sample preparation, Stable isotope labeling",
author = "Ping Yin and Xiaowen Hou and Romanova, {Elena Vikentievna} and Sweedler, {Jonathan V}",
year = "2011",
month = "10",
day = "24",
doi = "10.1007/978-1-61779-310-3_14",
language = "English (US)",
isbn = "9781617793097",
series = "Methods in Molecular Biology",
pages = "223--236",
editor = "Adalberto Merighi",
booktitle = "Neuropeptides",

}

TY - CHAP

T1 - Neuropeptidomics

T2 - Mass spectrometry-based qualitative and quantitative analysis

AU - Yin, Ping

AU - Hou, Xiaowen

AU - Romanova, Elena Vikentievna

AU - Sweedler, Jonathan V

PY - 2011/10/24

Y1 - 2011/10/24

N2 - Neuropeptidomics refers to a global characterization approach for the investigation of neuropeptides, often under specific physiological conditions. Neuropeptides comprise a complex set of signaling molecules that are involved in regulatory functions and behavioral control in the nervous system. Neuropeptidomics is inherently challenging because neuropeptides are spatially, temporally, and chemically heterogeneous, making them difficult to predict in silico from genomic information. Mature neuropeptides are produced from intricate enzymatic processing of precursor proteins/prohormones via a range of posttranslational modifications, resulting in multiple final peptide products from each prohormone gene. Although there are several methods for targeted peptide studies, mass spectrometry (MS), with its qualitative and quantitative capabilities, is ideally suited to the task. MS provides fast, sensitive, accurate, and high-throughput peptidomic analysis of neuropeptides without requiring prior knowledge of the peptide sequences. Aided by liquid chromatography (LC) separations and bioinformatics, MS is quickly becoming a leading technique in neuropeptidomics. This chapter describes several LC-MS analytical methods to identify, characterize, and quantify neuropeptides while emphasizing the sample preparation steps so integral to experimental success.

AB - Neuropeptidomics refers to a global characterization approach for the investigation of neuropeptides, often under specific physiological conditions. Neuropeptides comprise a complex set of signaling molecules that are involved in regulatory functions and behavioral control in the nervous system. Neuropeptidomics is inherently challenging because neuropeptides are spatially, temporally, and chemically heterogeneous, making them difficult to predict in silico from genomic information. Mature neuropeptides are produced from intricate enzymatic processing of precursor proteins/prohormones via a range of posttranslational modifications, resulting in multiple final peptide products from each prohormone gene. Although there are several methods for targeted peptide studies, mass spectrometry (MS), with its qualitative and quantitative capabilities, is ideally suited to the task. MS provides fast, sensitive, accurate, and high-throughput peptidomic analysis of neuropeptides without requiring prior knowledge of the peptide sequences. Aided by liquid chromatography (LC) separations and bioinformatics, MS is quickly becoming a leading technique in neuropeptidomics. This chapter describes several LC-MS analytical methods to identify, characterize, and quantify neuropeptides while emphasizing the sample preparation steps so integral to experimental success.

KW - Liquid chromatography

KW - Mass spectrometry

KW - Peptide identification

KW - Quantitation

KW - Sample preparation

KW - Stable isotope labeling

UR - http://www.scopus.com/inward/record.url?scp=80054773169&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054773169&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-310-3_14

DO - 10.1007/978-1-61779-310-3_14

M3 - Chapter

C2 - 21922411

AN - SCOPUS:80054773169

SN - 9781617793097

T3 - Methods in Molecular Biology

SP - 223

EP - 236

BT - Neuropeptides

A2 - Merighi, Adalberto

ER -