Neuronal leucine-rich repeat 6 (XlNLRR-6) is required for late lens and retina development in Xenopus laevis

Adam D. Wolfe, Jonathan J. Henry

Research output: Contribution to journalArticle

Abstract

Leucine-rich repeat proteins expressed in the developing vertebrate nervous system comprise a complex, multifamily group, and little is known of their developmental function in vivo. We have identified a novel member of this group in Xenopus laevis, XlNLRR-6, and through sequence and phylogenetic analysis, have placed it within a defined family of vertebrate neuronal leucine-rich repeat proteins (NLRR). XlNLRR-6 is expressed in the developing nervous system and tissues of the eye beginning at the neural plate stage, and expression continues throughout embryonic and larval development. Using antisense morpholino oligonucleotide (MO) -mediated knockdown of XlNLRR-6, we demonstrate that this protein is critical for development of the lens, retina, and cornea. Reciprocal transplantation of presumptive lens ectoderm between MO-treated and untreated embryos demonstrate that XlNLRR-6 plays autonomous roles in the development of both the lens and retina. These findings represent the first in vivo functional analysis of an NLRR family protein and establish a role for this protein during late differentiation of tissues in the developing eye.

Original languageEnglish (US)
Pages (from-to)1027-1041
Number of pages15
JournalDevelopmental Dynamics
Volume235
Issue number4
DOIs
StatePublished - Apr 1 2006

Fingerprint

Xenopus laevis
Leucine
Lenses
Retina
Morpholinos
Nervous System
Vertebrates
Neural Plate
Nerve Tissue
Ectoderm
Proteins
Antisense Oligonucleotides
Cornea
Embryonic Development
Sequence Analysis
Embryonic Structures
Transplantation
leucine-rich repeat proteins

Keywords

  • Cornea
  • Lens
  • Morpholino
  • Neuronal leucine rich repeat protein
  • Retina
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Neuronal leucine-rich repeat 6 (XlNLRR-6) is required for late lens and retina development in Xenopus laevis. / Wolfe, Adam D.; Henry, Jonathan J.

In: Developmental Dynamics, Vol. 235, No. 4, 01.04.2006, p. 1027-1041.

Research output: Contribution to journalArticle

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