Netrin-1 signaling regulates de novo protein synthesis of κ opioid receptor by facilitating polysomal partition of its mRNA

Nien Pei Tsai, Jing Bi, Horace H. Loh, Li Na Wei

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of κ opioid receptor (KOR) is subjected to both transcriptional and posttranscriptional controls. We report that KOR translation is regulated by netrin-1 in primary neurons of dorsal root ganglion (DRG) and in P19 embryonal carcinoma cells. Without stimulation, a significant portion of KOR mRNA is maintained in a dormant state and partitions in the translationally inactive, postpolysomal fraction. During netrin-1 stimulation, which activates its downstream target focal adhesion kinase (FAK), KOR mRNA rapidly partitions to the translationally active polysomal fraction. Functionally, the newly synthesized KOR proteins in DRG neurons are able to bind to specific ligands. This report describes the first example of netrin-1 signaling in the translational control of a drug receptor KOR, which involves the mediator of netrin-1, FAK, and a novel mechanism that enhances the association of target mRNA with polysomes for translational activation.

Original languageEnglish (US)
Pages (from-to)9743-9749
Number of pages7
JournalJournal of Neuroscience
Volume26
Issue number38
DOIs
StatePublished - Sep 20 2006
Externally publishedYes

Keywords

  • Dorsal root ganglia neuron
  • FAK
  • Netrin-1
  • Polysomal partition
  • Translational regulation
  • κ opioid receptor

ASJC Scopus subject areas

  • Neuroscience(all)

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