Abstract
Nerve growth factor-β (NGF) is a seminal plasma protein associated with improved sperm membrane integrity and motility in mammalian species. The objective of this study was to compare post-thaw semen quality from both ejaculated and pididymal-collected bull sperm incubated with purified NGF prior to cryopreservation. Semen was obtained from Angus × Simmental crossbred bulls (n = 10) collected by electroejaculation, followed by castration and epididymal sperm collections 3 days later. Semen samples were incubated with extender having 0 ng/mL (CONT), 0.5 ng/mL (LOW), 5 ng/mL (MED), or 50 ng/mL (HIGH) of purified NGF prior to cryopreservation. Sperm motility was assessed in each sample prior to treatment and cryopreservation and at post-thaw. Flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). Values for post-thaw sperm motility and velocity variables were decreased, while linearity was increased in samples of the HIGH compared with CONT group (P < 0.01), but there were no differences in epididymal samples (P> 0.05). Samples from the HIGH group also had a lesser amplitude of lateral head displacement at 2.5 and 3 h post-thaw (P < 0.01). Post-thaw sperm viability, acrosome integrity, and DNA fragmentation index were not affected by NGF treatment in either ejaculated or epididymal sperm (P> 0.05). In conclusion, supplementation of freezing extender with NGF had minimal effects on post-thaw sperm quality in bulls. Results indicate NGF may have a function in preventing premature sperm hyperactivation in ejaculated, but not epididymal-collected spermatozoa. Fertility studies, both in vitro and in vivo, are warranted to ascertain the relevancy of these findings.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 107-117 |
| Number of pages | 11 |
| Journal | Animal reproduction science |
| Volume | 207 |
| DOIs | |
| State | Published - Aug 2019 |
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Keywords
- Bovine
- Flow cytometry
- Motility
- Nerve growth factor-beta
- Spermatozoa
ASJC Scopus subject areas
- Food Animals
- Animal Science and Zoology
- Endocrinology
Cite this
Nerve growth factor-β effects on post-thaw bull semen quality : Effects of nerve growth factor-β added to extenders for cryopreservation of electro-ejaculated and epididymal bull semen. / Stewart, Jamie L.; Frederico Canisso, Igor; Podico, Giorgia; Kaplan, Claire; Garrett, Edgar F.; Shike, Daniel William; Henley, Parker; Lima, Fabio.
In: Animal reproduction science, Vol. 207, 08.2019, p. 107-117.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nerve growth factor-β effects on post-thaw bull semen quality
T2 - Effects of nerve growth factor-β added to extenders for cryopreservation of electro-ejaculated and epididymal bull semen
AU - Stewart, Jamie L.
AU - Frederico Canisso, Igor
AU - Podico, Giorgia
AU - Kaplan, Claire
AU - Garrett, Edgar F.
AU - Shike, Daniel William
AU - Henley, Parker
AU - Lima, Fabio
PY - 2019/8
Y1 - 2019/8
N2 - Nerve growth factor-β (NGF) is a seminal plasma protein associated with improved sperm membrane integrity and motility in mammalian species. The objective of this study was to compare post-thaw semen quality from both ejaculated and pididymal-collected bull sperm incubated with purified NGF prior to cryopreservation. Semen was obtained from Angus × Simmental crossbred bulls (n = 10) collected by electroejaculation, followed by castration and epididymal sperm collections 3 days later. Semen samples were incubated with extender having 0 ng/mL (CONT), 0.5 ng/mL (LOW), 5 ng/mL (MED), or 50 ng/mL (HIGH) of purified NGF prior to cryopreservation. Sperm motility was assessed in each sample prior to treatment and cryopreservation and at post-thaw. Flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). Values for post-thaw sperm motility and velocity variables were decreased, while linearity was increased in samples of the HIGH compared with CONT group (P < 0.01), but there were no differences in epididymal samples (P> 0.05). Samples from the HIGH group also had a lesser amplitude of lateral head displacement at 2.5 and 3 h post-thaw (P < 0.01). Post-thaw sperm viability, acrosome integrity, and DNA fragmentation index were not affected by NGF treatment in either ejaculated or epididymal sperm (P> 0.05). In conclusion, supplementation of freezing extender with NGF had minimal effects on post-thaw sperm quality in bulls. Results indicate NGF may have a function in preventing premature sperm hyperactivation in ejaculated, but not epididymal-collected spermatozoa. Fertility studies, both in vitro and in vivo, are warranted to ascertain the relevancy of these findings.
AB - Nerve growth factor-β (NGF) is a seminal plasma protein associated with improved sperm membrane integrity and motility in mammalian species. The objective of this study was to compare post-thaw semen quality from both ejaculated and pididymal-collected bull sperm incubated with purified NGF prior to cryopreservation. Semen was obtained from Angus × Simmental crossbred bulls (n = 10) collected by electroejaculation, followed by castration and epididymal sperm collections 3 days later. Semen samples were incubated with extender having 0 ng/mL (CONT), 0.5 ng/mL (LOW), 5 ng/mL (MED), or 50 ng/mL (HIGH) of purified NGF prior to cryopreservation. Sperm motility was assessed in each sample prior to treatment and cryopreservation and at post-thaw. Flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). Values for post-thaw sperm motility and velocity variables were decreased, while linearity was increased in samples of the HIGH compared with CONT group (P < 0.01), but there were no differences in epididymal samples (P> 0.05). Samples from the HIGH group also had a lesser amplitude of lateral head displacement at 2.5 and 3 h post-thaw (P < 0.01). Post-thaw sperm viability, acrosome integrity, and DNA fragmentation index were not affected by NGF treatment in either ejaculated or epididymal sperm (P> 0.05). In conclusion, supplementation of freezing extender with NGF had minimal effects on post-thaw sperm quality in bulls. Results indicate NGF may have a function in preventing premature sperm hyperactivation in ejaculated, but not epididymal-collected spermatozoa. Fertility studies, both in vitro and in vivo, are warranted to ascertain the relevancy of these findings.
KW - Bovine
KW - Flow cytometry
KW - Motility
KW - Nerve growth factor-beta
KW - Spermatozoa
UR - http://www.scopus.com/inward/record.url?scp=85067203943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067203943&partnerID=8YFLogxK
U2 - 10.1016/j.anireprosci.2019.06.010
DO - 10.1016/j.anireprosci.2019.06.010
M3 - Article
C2 - 31204090
AN - SCOPUS:85067203943
VL - 207
SP - 107
EP - 117
JO - Animal Reproduction Science
JF - Animal Reproduction Science
SN - 0378-4320
ER -