@article{8f01d7823dbe4698b9f80b30bd329045,
title = "Nephroblastoma in a Common Mudpuppy Necturus maculosus simultaneously Present with a Mollicute Bacterium of the Genus Acholeplasma",
abstract = "In March 2017, a wild-caught female common mudpuppy Necturus maculosus from Iowa, USA, with an enlarged posterior abdomen was submitted for diagnostic assessment. The cause of the abdominal distension was a large fluid-filled abdominal mass, diagnosed as a nephroblastoma. Parasites and numerous bacteria were isolated and identified from the mudpuppy but were determined to be incidental. Samples of the neoplasm inoculated onto an American toad Anaxyrus americanus cell line (BufoTad) yielded cytopathic effect during several passages. However, standard molecular testing of the cell culture supernatant failed to identify any viruses. Next-generation sequencing identified the replicating agent as a bacterium of the genus Acholeplasma. Immunohistochemistry confirmed the presence of Acholeplasma within the nephroblastoma, including within tumor cells. This is the first report of nephroblastoma and the second report of neoplasia in this species. The results also suggest that certain bacteria of the genus Acholeplasma might be oncogenic.",
author = "Isaac Standish and Eric Leis and Sara Erickson and Ryan Katona and Wes Baumgartner and Kevin Hanson and Iman Ibrahim and Tony Goldberg",
note = "Funding Information: The authors thank the USFWS staff and coworkers at the Midwest Fisheries Center and the National Conservation Training Center (NCTC) for their assistance and motivation, John Fisher (USFWS, NCTC) for his assistance in obtaining literature and publications, and Christopher Dunn for assistance with next‐generation sequencing. This research was funded in part by the University of Wisconsin–Madison John D. MacArthur Fellowship Program. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the USFWS or U.S. Government. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the USFWS or U.S. Government. There is no conflict of interest declared in this article. Funding Information: The authors thank the USFWS staff and coworkers at the Midwest Fisheries Center and the National Conservation Training Center (NCTC) for their assistance and motivation, John Fisher (USFWS, NCTC) for his assistance in obtaining literature and publications, and Christopher Dunn for assistance with next-generation sequencing. This research was funded in part by the University of Wisconsin–Madison John D. MacArthur Fellowship Program. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the USFWS or U.S. Government. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the USFWS or U.S. Government. There is no conflict of interest declared in this article. Publisher Copyright: {\textcopyright} 2021 American Fisheries Society",
year = "2021",
month = mar,
doi = "10.1002/aah.10119",
language = "English (US)",
volume = "33",
pages = "44--52",
journal = "Journal of Aquatic Animal Health",
issn = "0899-7659",
publisher = "American Fisheries Society",
number = "1",
}