Near-infrared photoactivatable nitric oxide donors with photoacoustic readout

Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo, Jefferson Chan

Research output: Chapter in Book/Report/Conference proceedingChapter


In this chapter, we motivate the need for photoactivatable NO donor molecules and give a brief survey of the existing chemical tools in the field. We then provide detailed protocols for the synthesis and validation of a near-infrared light-activated NO donor molecule, photoNOD-1, developed in our research group. With this tool, NO can be released in vivo in a radiation-dependent manner that can be monitored using photoacoustic imaging.

Original languageEnglish (US)
Title of host publicationChemical Tools for Imaging, Manipulating, and Tracking Biological Systems
Subtitle of host publicationDiverse Chemical, Optical and Bioorthogonal Methods
EditorsDavid M. Chenoweth
PublisherAcademic Press Inc.
Number of pages35
ISBN (Print)9780128211564
StatePublished - 2020

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Donors
  • Near-infrared
  • Nitric oxide
  • Photoacoustic imaging
  • Photoactivation
  • Real-time monitoring

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry


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