Abstract
We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.
Original language | English (US) |
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Pages (from-to) | 303-309 |
Number of pages | 7 |
Journal | Ultramicroscopy |
Volume | 71 |
Issue number | 1-4 |
DOIs | |
State | Published - Mar 1 1998 |
Externally published | Yes |
Event | Proceedings of the 1997 4th International Conference on Near-Field Optics and Related Techniques, NFO-4 - Jerusalem, Israel Duration: Feb 9 1997 → Feb 13 1997 |
Keywords
- Green fluorescent protein
- Malaria
- Microscopic methods, specifically for biological specimens
- Near-field optical microscopy
- Red- blood cells
- Spectroscopy in NFOM
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Instrumentation