Naturally amplified player for biosensing: tmRNA to the rescue

Ott Scheler; Lauris Kaplinski; Barry Glynn; Ryan C. Bailey; Ants Kurg

doi:10.1016/j.proeng.2011.12.383

Naturally amplified player for biosensing: tmRNA to the rescue

Ott Scheler, Lauris Kaplinski, Barry Glynn, Ryan C. Bailey, Ants Kurg

Chemistry

Research output: Contribution to journal › Conference article › peer-review

Abstract

We present a trans-translation mediating tmRNA molecule from bacteria that can be used as a marker in microbial diagnostics and/or taxonomical studies. Different bacteria can be detected and specifically identified using surface bound complementary DNA oligonucleotide probes on different sensor platforms. Several tmRNA hybridization techniques were studied on microarray and in real-time on SOI microring resonator biosensor platforms. Microarray based detection technology enabled detection of tmRNA molecules from less than 1 cell equivalent of pathogenic bacteria.

Original language	English (US)
Pages (from-to)	1549-1552
Number of pages	4
Journal	Procedia Engineering
Volume	25
DOIs	https://doi.org/10.1016/j.proeng.2011.12.383
State	Published - 2011
Event	25th Eurosensors Conference - Athens, Greece Duration: Sep 4 2011 → Sep 7 2011

Keywords

Microarray
Molecular diagnostics
NASBA
SOI microring resonators
TmRNA

ASJC Scopus subject areas

General Engineering

Online availability

10.1016/j.proeng.2011.12.383

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title = "Naturally amplified player for biosensing: tmRNA to the rescue",

abstract = "We present a trans-translation mediating tmRNA molecule from bacteria that can be used as a marker in microbial diagnostics and/or taxonomical studies. Different bacteria can be detected and specifically identified using surface bound complementary DNA oligonucleotide probes on different sensor platforms. Several tmRNA hybridization techniques were studied on microarray and in real-time on SOI microring resonator biosensor platforms. Microarray based detection technology enabled detection of tmRNA molecules from less than 1 cell equivalent of pathogenic bacteria.",

keywords = "Microarray, Molecular diagnostics, NASBA, SOI microring resonators, TmRNA",

author = "Ott Scheler and Lauris Kaplinski and Barry Glynn and Bailey, {Ryan C.} and Ants Kurg",

note = "Funding Information: This research was supported by European Social Fund{\textquoteright}s Doctoral Studies and Internationalisation Programme DoRa and targeted financing SF0180026s09 and SF0180027s10 from the Estonian Ministry of Education and Research. Financial support is also acknowledged from the United States National Institutes of Health (NIH) New Innovator Award Program, part of the NIH Roadmap for Medical Research, through grant number 1-DP2-OD002109-01; 25th Eurosensors Conference ; Conference date: 04-09-2011 Through 07-09-2011",

year = "2011",

doi = "10.1016/j.proeng.2011.12.383",

language = "English (US)",

volume = "25",

pages = "1549--1552",

journal = "Procedia Engineering",

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TY - JOUR

T1 - Naturally amplified player for biosensing

T2 - 25th Eurosensors Conference

AU - Scheler, Ott

AU - Kaplinski, Lauris

AU - Glynn, Barry

AU - Bailey, Ryan C.

AU - Kurg, Ants

N1 - Funding Information: This research was supported by European Social Fund’s Doctoral Studies and Internationalisation Programme DoRa and targeted financing SF0180026s09 and SF0180027s10 from the Estonian Ministry of Education and Research. Financial support is also acknowledged from the United States National Institutes of Health (NIH) New Innovator Award Program, part of the NIH Roadmap for Medical Research, through grant number 1-DP2-OD002109-01

PY - 2011

Y1 - 2011

N2 - We present a trans-translation mediating tmRNA molecule from bacteria that can be used as a marker in microbial diagnostics and/or taxonomical studies. Different bacteria can be detected and specifically identified using surface bound complementary DNA oligonucleotide probes on different sensor platforms. Several tmRNA hybridization techniques were studied on microarray and in real-time on SOI microring resonator biosensor platforms. Microarray based detection technology enabled detection of tmRNA molecules from less than 1 cell equivalent of pathogenic bacteria.

AB - We present a trans-translation mediating tmRNA molecule from bacteria that can be used as a marker in microbial diagnostics and/or taxonomical studies. Different bacteria can be detected and specifically identified using surface bound complementary DNA oligonucleotide probes on different sensor platforms. Several tmRNA hybridization techniques were studied on microarray and in real-time on SOI microring resonator biosensor platforms. Microarray based detection technology enabled detection of tmRNA molecules from less than 1 cell equivalent of pathogenic bacteria.

KW - Microarray

KW - Molecular diagnostics

KW - NASBA

KW - SOI microring resonators

KW - TmRNA

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U2 - 10.1016/j.proeng.2011.12.383

DO - 10.1016/j.proeng.2011.12.383

M3 - Conference article

AN - SCOPUS:84857176324

SN - 1877-7058

VL - 25

SP - 1549

EP - 1552

JO - Procedia Engineering

JF - Procedia Engineering

Y2 - 4 September 2011 through 7 September 2011

ER -

Naturally amplified player for biosensing: tmRNA to the rescue

Abstract

Keywords

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