Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA

Xinying Zong, Shinichi Nakagawa, Susan M. Freier, Jingyi Fei, Taekjip Ha, Supriya G. Prasanth, Kannanganattu V. Prasanth

Research output: Contribution to journalArticle

Abstract

The RNase P-mediated endonucleolytic cleavage plays a crucial role in the 3′ end processing and cellular accumulation of MALAT1, a nuclear-retained long noncoding RNA that promotes malignancy. The regulation of this cleavage event is largely undetermined. Here we characterize a broadly expressed natural antisense transcript at the MALAT1 locus, designated as TALAM1, that positively regulates MALAT1 levels by promoting the 3′ end cleavage and maturation of MALAT1 RNA. TALAM1 RNA preferentially localizes at the site of transcription, and also interacts with MALAT1 RNA. Depletion of TALAM1 leads to defects in the 3′ end cleavage reaction and compromises cellular accumulation of MALAT1. Conversely, overexpression of TALAM1 facilitates the cleavage reaction in trans. Interestingly, TALAM1 is also positively regulated by MALAT1 at the level of both transcription and RNA stability. Together, our data demonstrate a novel feed-forward positive regulatory loop that is established to maintain the high cellular levels of MALAT1, and also unravel the existence of sense-antisense mediated regulatory mechanism for cellular lncRNAs that display RNase P-mediated 3′ end processing.

Original languageEnglish (US)
Pages (from-to)2898-2908
Number of pages11
JournalNucleic acids research
Volume44
Issue number6
DOIs
StatePublished - Jan 29 2016

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RNA 3' End Processing
Long Noncoding RNA
Antisense RNA
Ribonuclease P
RNA
RNA Stability
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA. / Zong, Xinying; Nakagawa, Shinichi; Freier, Susan M.; Fei, Jingyi; Ha, Taekjip; Prasanth, Supriya G.; Prasanth, Kannanganattu V.

In: Nucleic acids research, Vol. 44, No. 6, 29.01.2016, p. 2898-2908.

Research output: Contribution to journalArticle

Zong, Xinying ; Nakagawa, Shinichi ; Freier, Susan M. ; Fei, Jingyi ; Ha, Taekjip ; Prasanth, Supriya G. ; Prasanth, Kannanganattu V. / Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA. In: Nucleic acids research. 2016 ; Vol. 44, No. 6. pp. 2898-2908.
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