TY - JOUR
T1 - Narrowing down the region of the Vf locus for scab resistance in apple using AFLP-derived SCARs
AU - Huaracha, E.
AU - Xu, M.
AU - Korban, S. S.
PY - 2004/1
Y1 - 2004/1
N2 - A narrow-down strategy to restrict the Vf region, which controls resistance to the fungal disease apple scab in apple, to a genetic distance of 0.4 cM is presented. Using 11 AFLP-derived SCARs and three RAPD-derived SCARs, all linked to the Vf gene, we subjected 1,412 scab-resistant individuals from 16 mapping populations to genotype analysis. Eleven recombinant individuals were identified within a genetic distance of 0.9 cM around the Vf gene. Using these 11 recombinants, we achieved fine-resolution of several AFLP-derived SCAR markers surrounding the Vf gene, resulting in the following genetic linkage map: ACS-6 and ACS are located left of the Vf gene at genetic distances of 0.2 cM and 0.1 cM, respectively; ACS-7 and ACS-9 are inseparable from the Vf gene; ACS-8, ACS-10, and ACS-4 are located to the right of the Vf gene at genetic distances of 0.1 cM, 0.4 cM, and 0.5 cM, respectively; the remaining five SCARs - ACS-11, ACS-5, ACS-2, ACS-1, and AL07 - are inseparable and are located right of the Vf gene at a genetic distance of 0.7 cM. By integrating this linkage data with our previous physical map, we generated a revised map of the narrowed-down region of Vf.
AB - A narrow-down strategy to restrict the Vf region, which controls resistance to the fungal disease apple scab in apple, to a genetic distance of 0.4 cM is presented. Using 11 AFLP-derived SCARs and three RAPD-derived SCARs, all linked to the Vf gene, we subjected 1,412 scab-resistant individuals from 16 mapping populations to genotype analysis. Eleven recombinant individuals were identified within a genetic distance of 0.9 cM around the Vf gene. Using these 11 recombinants, we achieved fine-resolution of several AFLP-derived SCAR markers surrounding the Vf gene, resulting in the following genetic linkage map: ACS-6 and ACS are located left of the Vf gene at genetic distances of 0.2 cM and 0.1 cM, respectively; ACS-7 and ACS-9 are inseparable from the Vf gene; ACS-8, ACS-10, and ACS-4 are located to the right of the Vf gene at genetic distances of 0.1 cM, 0.4 cM, and 0.5 cM, respectively; the remaining five SCARs - ACS-11, ACS-5, ACS-2, ACS-1, and AL07 - are inseparable and are located right of the Vf gene at a genetic distance of 0.7 cM. By integrating this linkage data with our previous physical map, we generated a revised map of the narrowed-down region of Vf.
KW - AFLP
KW - Apple
KW - Apple scab disease
KW - SCAR
KW - Vf gene
UR - http://www.scopus.com/inward/record.url?scp=0842287595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0842287595&partnerID=8YFLogxK
U2 - 10.1007/s00122-003-1435-5
DO - 10.1007/s00122-003-1435-5
M3 - Article
C2 - 13679976
AN - SCOPUS:0842287595
SN - 0040-5752
VL - 108
SP - 274
EP - 279
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 2
ER -