Narrowing down the region of the Vf locus for scab resistance in apple using AFLP-derived SCARs

E. Huaracha, M. Xu, S. S. Korban

Research output: Contribution to journalArticlepeer-review

Abstract

A narrow-down strategy to restrict the Vf region, which controls resistance to the fungal disease apple scab in apple, to a genetic distance of 0.4 cM is presented. Using 11 AFLP-derived SCARs and three RAPD-derived SCARs, all linked to the Vf gene, we subjected 1,412 scab-resistant individuals from 16 mapping populations to genotype analysis. Eleven recombinant individuals were identified within a genetic distance of 0.9 cM around the Vf gene. Using these 11 recombinants, we achieved fine-resolution of several AFLP-derived SCAR markers surrounding the Vf gene, resulting in the following genetic linkage map: ACS-6 and ACS are located left of the Vf gene at genetic distances of 0.2 cM and 0.1 cM, respectively; ACS-7 and ACS-9 are inseparable from the Vf gene; ACS-8, ACS-10, and ACS-4 are located to the right of the Vf gene at genetic distances of 0.1 cM, 0.4 cM, and 0.5 cM, respectively; the remaining five SCARs - ACS-11, ACS-5, ACS-2, ACS-1, and AL07 - are inseparable and are located right of the Vf gene at a genetic distance of 0.7 cM. By integrating this linkage data with our previous physical map, we generated a revised map of the narrowed-down region of Vf.

Original languageEnglish (US)
Pages (from-to)274-279
Number of pages6
JournalTheoretical and Applied Genetics
Volume108
Issue number2
DOIs
StatePublished - Jan 1 2004

Keywords

  • AFLP
  • Apple
  • Apple scab disease
  • SCAR
  • Vf gene

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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