Nanostructured optical photonic crystal biosensor for HIV viral load measurement

Hadi Shafiee, Erich A. Lidstone, Muntasir Jahangir, Fatih Inci, Emily Hanhauser, Timothy J. Henrich, Daniel R. Kuritzkes, Brian T. Cunningham, Utkan Demirci

Research output: Contribution to journalArticlepeer-review

Abstract

Detecting and quantifying biomarkers and viruses in biological samples have broad applications in early disease diagnosis and treatment monitoring. We have demonstrated a label-free optical sensing mechanism using nanostructured photonic crystals (PC) to capture and quantify intact viruses (HIV-1) from biologically relevant samples. The nanostructured surface of the PC biosensor resonantly reflects a narrow wavelength band during illumination with a broadband light source. Surface-adsorbed biotarget induces a shift in the resonant Peak Wavelength Value (PWV) that is detectable with <10pm wavelength resolution, enabling detection of both biomolecular layers and small number of viruses that sparsely populate the transducer surface. We have successfully captured and detected HIV-1 in serum and phosphate buffered saline (PBS) samples with viral loads ranging from 104 to 108 copies/mL. The surface density of immobilized biomolecular layers used in the sensor functionalization process, including 3-mercaptopropyltrimethoxysilane (3-MPS), N-gamma-Maleimidobutyryl-oxysuccinimide ester (GMBS), NeutrAvidin, anti-gp120, and bovine serum albumin (BSA) were also quantified by the PC biosensor.

Original languageEnglish (US)
Article number4116
JournalScientific reports
Volume4
DOIs
StatePublished - Feb 28 2014

ASJC Scopus subject areas

  • General

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