Abstract
Atomic Force Microscopy-Infrared spectroscopy (AFM-IR) is a powerful technique for mapping material composition at sub micrometer length scales. Unlike related technologies such as scanning near-field optical microscopy (SNOM), the detected signal can be equally sensitive to both chemical and mechanical sample properties due to variations in the cantilever's response (or responsivity) to a sample perturbation local to the tip. Understanding cantilever responsivity has led to new approaches for correcting this effect, which show improved chemical specificity and imaging fidelity for heterogeneous samples. Here, we present implications of correcting the cantilever responsivity effect in AFM-IR images for studying biological samples at nanometer length scales and discuss the future of this emerging technology.
| Original language | English (US) |
|---|---|
| Title of host publication | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI |
| Editors | Dan V. Nicolau, Dror Fixler, Ewa M. Goldys |
| Publisher | SPIE |
| ISBN (Electronic) | 9781510624245 |
| DOIs | |
| State | Published - Jan 1 2019 |
| Event | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 - San Francisco, United States Duration: Feb 3 2019 → Feb 4 2019 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 10891 |
| ISSN (Print) | 1605-7422 |
Conference
| Conference | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 2/3/19 → 2/4/19 |
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Keywords
- AFM
- AFM-IR
- Infrared
- QCL
- Spectroscopy
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Nanoscale imaging of biological samples with responsivity corrected atomic force microscopy-infrared (AFM-IR) spectroscopy. / Kenkel, Seth; Bhargava, Rohit.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI. ed. / Dan V. Nicolau; Dror Fixler; Ewa M. Goldys. SPIE, 2019. 108910A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10891).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Nanoscale imaging of biological samples with responsivity corrected atomic force microscopy-infrared (AFM-IR) spectroscopy
AU - Kenkel, Seth
AU - Bhargava, Rohit
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Atomic Force Microscopy-Infrared spectroscopy (AFM-IR) is a powerful technique for mapping material composition at sub micrometer length scales. Unlike related technologies such as scanning near-field optical microscopy (SNOM), the detected signal can be equally sensitive to both chemical and mechanical sample properties due to variations in the cantilever's response (or responsivity) to a sample perturbation local to the tip. Understanding cantilever responsivity has led to new approaches for correcting this effect, which show improved chemical specificity and imaging fidelity for heterogeneous samples. Here, we present implications of correcting the cantilever responsivity effect in AFM-IR images for studying biological samples at nanometer length scales and discuss the future of this emerging technology.
AB - Atomic Force Microscopy-Infrared spectroscopy (AFM-IR) is a powerful technique for mapping material composition at sub micrometer length scales. Unlike related technologies such as scanning near-field optical microscopy (SNOM), the detected signal can be equally sensitive to both chemical and mechanical sample properties due to variations in the cantilever's response (or responsivity) to a sample perturbation local to the tip. Understanding cantilever responsivity has led to new approaches for correcting this effect, which show improved chemical specificity and imaging fidelity for heterogeneous samples. Here, we present implications of correcting the cantilever responsivity effect in AFM-IR images for studying biological samples at nanometer length scales and discuss the future of this emerging technology.
KW - AFM
KW - AFM-IR
KW - Infrared
KW - QCL
KW - Spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85064411868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064411868&partnerID=8YFLogxK
U2 - 10.1117/12.2510131
DO - 10.1117/12.2510131
M3 - Conference contribution
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI
A2 - Nicolau, Dan V.
A2 - Fixler, Dror
A2 - Goldys, Ewa M.
PB - SPIE
ER -