Abstract
It is hypothesized that the nanoscale organization of cell adhesion ligands in a synthetic ECM regulates nonviral gene delivery. This hypothesis was examined with pre-osteoblasts cultured on substrates which present varied density and spacing of synthetic adhesion ligands. The levels of gene transfer and expression were increased with the density of adhesion ligands, but decreased with the spacing of ligands, due to changes in the cell growth rate. This study provides a material-based control point on the nanometer scale for nonviral gene based therapies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 161-166 |
| Number of pages | 6 |
| Journal | Nano letters |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2007 |
ASJC Scopus subject areas
- Bioengineering
- Chemistry(all)
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering