TY - JOUR
T1 - Nanopore sensors for nucleic acid analysis
AU - Venkatesan, Bala Murali
AU - Bashir, Rashid
N1 - Funding Information:
B.M.V. is a trainee supported by the Midwestern Cancer Nanotechnology Training Center (NIH-NCI R25 CA154015). Support from the National Institutes of Health (R21 CA155863) and the National Science Foundation (EEC-0425626) is also acknowledged. The authors thank J. Hanlon-Sinn (Beckman Institute of Advanced Technology, University of Illinois at Urbana-Champaign) for the images in Fig. 6, and M. Drndic (University of Pennsylvania) for valuable discussions.
PY - 2010/10
Y1 - 2010/10
N2 - Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
AB - Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
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U2 - 10.1038/nnano.2011.129
DO - 10.1038/nnano.2011.129
M3 - Review article
C2 - 21926981
AN - SCOPUS:80455173836
VL - 6
SP - 615
EP - 624
JO - Nature Nanotechnology
JF - Nature Nanotechnology
SN - 1748-3387
IS - 10
ER -