Abstract
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 615-624 |
| Number of pages | 10 |
| Journal | Nature Nanotechnology |
| Volume | 6 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2010 |
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ASJC Scopus subject areas
- Bioengineering
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
- Materials Science(all)
- Condensed Matter Physics
- Electrical and Electronic Engineering
Cite this
Nanopore sensors for nucleic acid analysis. / Venkatesan, Bala Murali; Bashir, Rashid.
In: Nature Nanotechnology, Vol. 6, No. 10, 10.2010, p. 615-624.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Nanopore sensors for nucleic acid analysis
AU - Venkatesan, Bala Murali
AU - Bashir, Rashid
PY - 2010/10
Y1 - 2010/10
N2 - Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
AB - Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
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U2 - 10.1038/nnano.2011.129
DO - 10.1038/nnano.2011.129
M3 - Review article
C2 - 21926981
AN - SCOPUS:80455173836
VL - 6
SP - 615
EP - 624
JO - Nature Nanotechnology
JF - Nature Nanotechnology
SN - 1748-3387
IS - 10
ER -