Abstract

Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.

Original languageEnglish (US)
Pages (from-to)615-624
Number of pages10
JournalNature Nanotechnology
Volume6
Issue number10
DOIs
StatePublished - Oct 2010

Fingerprint

Nanopores
Nucleic acids
nucleic acids
Nucleic Acids
DNA
deoxyribonucleic acid
sequencing
sensors
Sensors
Molecules
Double-Stranded RNA
genome
Single-Stranded DNA
RNA
Electrolytes
Amplification
Labels
molecules
emerging
Polymers

ASJC Scopus subject areas

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Nanopore sensors for nucleic acid analysis. / Venkatesan, Bala Murali; Bashir, Rashid.

In: Nature Nanotechnology, Vol. 6, No. 10, 10.2010, p. 615-624.

Research output: Contribution to journalReview article

Venkatesan, Bala Murali ; Bashir, Rashid. / Nanopore sensors for nucleic acid analysis. In: Nature Nanotechnology. 2010 ; Vol. 6, No. 10. pp. 615-624.
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