Nanoplasmonic monitoring of odorants binding to olfactory proteins from honeybee as biosensor for chemical detection

Diming Zhang, Yanli Lu, Qian Zhang, Yao Yao, Shuang Li, Hongliang Li, Shulin Zhuang, Jing Jiang, Gang Logan Liu, Qingjun Liu

Research output: Contribution to journalArticlepeer-review


Abstract Odorant binding proteins (OBPs) are key soluble sensitive proteins for odor detections in animal olfactory systems. Here, we present a nanosensor based localized surface plasmon resonance (LSPR) to monitor binding of small odorant molecules to OBPs from honeybees. The binding of odorants molecules to OBPs, can be quantified by nanocup arrays from 10 nM to 1 mM, with a high refractive index sensing of LSPR wavelength shift in transmission spectra. Linear dose-dependent behavior can be observed in relationship of LSPR wavelength versus analyte concentrations. More than floral odorants, this type of bio-functionalized nanocups also showed potential applications in explosive detections for nitro-compounds such as 2,4,6-trinitrotoluene, 2,4-dinitrotoluene and 3-mononitrotoluene. This study demonstrated a LSPR device to monitor interactions between small molecules and proteins, which provided a great biosensor platform for healthcare diagnosis, environment monitoring and food analysis.

Original languageEnglish (US)
Article number18668
Pages (from-to)341-349
Number of pages9
JournalSensors and Actuators, B: Chemical
StatePublished - Jul 11 2015


  • Localized surface plasmon resonance (LSPR)
  • Nanocup arrays
  • Nitro-explosives
  • Odorant binding protein (OBP)
  • Small odorant molecules

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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