TY - JOUR
T1 - Nanoplasmonic monitoring of odorants binding to olfactory proteins from honeybee as biosensor for chemical detection
AU - Zhang, Diming
AU - Lu, Yanli
AU - Zhang, Qian
AU - Yao, Yao
AU - Li, Shuang
AU - Li, Hongliang
AU - Zhuang, Shulin
AU - Jiang, Jing
AU - Liu, Gang Logan
AU - Liu, Qingjun
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 81371643 ; No. 31372254 ) and the Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars (Grant No. LR13H180002 ).
PY - 2015/7/11
Y1 - 2015/7/11
N2 - Abstract Odorant binding proteins (OBPs) are key soluble sensitive proteins for odor detections in animal olfactory systems. Here, we present a nanosensor based localized surface plasmon resonance (LSPR) to monitor binding of small odorant molecules to OBPs from honeybees. The binding of odorants molecules to OBPs, can be quantified by nanocup arrays from 10 nM to 1 mM, with a high refractive index sensing of LSPR wavelength shift in transmission spectra. Linear dose-dependent behavior can be observed in relationship of LSPR wavelength versus analyte concentrations. More than floral odorants, this type of bio-functionalized nanocups also showed potential applications in explosive detections for nitro-compounds such as 2,4,6-trinitrotoluene, 2,4-dinitrotoluene and 3-mononitrotoluene. This study demonstrated a LSPR device to monitor interactions between small molecules and proteins, which provided a great biosensor platform for healthcare diagnosis, environment monitoring and food analysis.
AB - Abstract Odorant binding proteins (OBPs) are key soluble sensitive proteins for odor detections in animal olfactory systems. Here, we present a nanosensor based localized surface plasmon resonance (LSPR) to monitor binding of small odorant molecules to OBPs from honeybees. The binding of odorants molecules to OBPs, can be quantified by nanocup arrays from 10 nM to 1 mM, with a high refractive index sensing of LSPR wavelength shift in transmission spectra. Linear dose-dependent behavior can be observed in relationship of LSPR wavelength versus analyte concentrations. More than floral odorants, this type of bio-functionalized nanocups also showed potential applications in explosive detections for nitro-compounds such as 2,4,6-trinitrotoluene, 2,4-dinitrotoluene and 3-mononitrotoluene. This study demonstrated a LSPR device to monitor interactions between small molecules and proteins, which provided a great biosensor platform for healthcare diagnosis, environment monitoring and food analysis.
KW - Localized surface plasmon resonance (LSPR)
KW - Nanocup arrays
KW - Nitro-explosives
KW - Odorant binding protein (OBP)
KW - Small odorant molecules
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U2 - 10.1016/j.snb.2015.06.091
DO - 10.1016/j.snb.2015.06.091
M3 - Article
AN - SCOPUS:84936849928
VL - 221
SP - 341
EP - 349
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
M1 - 18668
ER -