Nanoplasmon coupled intracellular optical resonance excitation for ultrasensitive 3D fluorescence cell imaging

Manas Ranjan Gartia, Austin Hsiao, Mayandi Sivaguru, Yi Chen, Gang Logan Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have created an enhanced cell-imaging platform for 3D confocal fluorescence cell imaging where fluorescence sensitivity is amplified for more than 100 folds especially for cell membrane and cytoplasm. The observed unprecedented three-dimensional fluorescence intensity enhancement on the entire cell microstructure including cell membrane 10 μm above the substrate surface is attributed to a novel far field enhancement mechanism, nanoplasmon coupled optical resonance excitation (CORE) mechanism which permits enhanced surface plasmon on the substrate being evanescently coupled to Whispering Gallery mode optical resonance inside the spheroidal cell membrane microcavity. Theoretical model of the hypothesis is explained using coupled mode theory. In addition, preliminary result has been provided for the observation of early stage of transfection in a cancer cell.

Original languageEnglish (US)
Title of host publicationBiosensing and Nanomedicine IV
DOIs
StatePublished - 2011
Externally publishedYes
EventBiosensing and Nanomedicine IV - San Diego, CA, United States
Duration: Aug 21 2011Aug 23 2011

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8099
ISSN (Print)0277-786X

Other

OtherBiosensing and Nanomedicine IV
Country/TerritoryUnited States
CitySan Diego, CA
Period8/21/118/23/11

Keywords

  • Cell imaging
  • Confocal microscopy
  • Metal enhanced fluorescence
  • Surface plasmon

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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