@inproceedings{316276a6845f46389ecdf6fafc415d96,
title = "Nanomolecular histopathology for renal tumor classification",
abstract = "Renal tumors are classified into histopathologic subtypes based on light microscopy. Classification is important because subtypes have distinct genetic abnormalities and clinical behavior, yet is difficult because many cases have heterogeneous morphology. Thus, several molecular assays have been developed for tumor classification. Immunohistochemistry (IHC) permits correlation of molecular data with tissue morphology, but is difficult to quantify or perform in multiplex. Quantitative RT-PCR and gene expression microarrays are more quantitative, but lose morphologic information. We hypothesize that direct integration of histopathology with nanomolecular expression profiling will provide sensitive and specific data for renal tumor classification. Thus, we are developing novel IHC assays using antibodies conjugated to nanoparticle tags with surface-enhanced Raman scattering properties (SERS). Our preliminary data suggests that SERS-IHC can be performed using standard microscopic equipment, with potential for sensitive, quantitative multiplex assays on fixed tissues.",
author = "Young, {Andrew N.} and Amin, {Mahul B.} and Petros, {John A.} and Natan, {Michael J.} and Shuming Nie and Wang, {May D.}",
year = "2005",
doi = "10.1109/iembs.2005.1616516",
language = "English (US)",
isbn = "0780387406",
series = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "723--726",
booktitle = "Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005",
address = "United States",
note = "2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 ; Conference date: 01-09-2005 Through 04-09-2005",
}