Nanogyroids incorporating multivalent lipids: Enhanced membrane charge density and pore forming ability for gene silencing

Cecilia Leal, Kai K. Ewert, Rahau S. Shirazi, Nathan F. Bouxsein, Cyrus R. Safinya

Research output: Contribution to journalArticlepeer-review

Abstract

The self-assembly of a custom-synthesized pentavalent cationic lipid (MVL5) and glycerol monooleate (GMO) with small interfering RNA (siRNA) results in the formation of a double-gyroid bicontinuous inverted cubic phase with colocalized lipid/siRNA domains as shown by synchrotron X-ray scattering and fluorescence microscopy. The high charge density (due to MVL5) and positive Gaussian modulus of the GMO-containing membranes confer optimal electrostatic and elastic properties for endosomal escape, enabling efficient siRNA delivery and effective, specific gene silencing.

Original languageEnglish (US)
Pages (from-to)7691-7697
Number of pages7
JournalLangmuir
Volume27
Issue number12
DOIs
StatePublished - Jun 21 2011
Externally publishedYes

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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