Nanofluidic interconnects within a multilayer microfluidic chip for attomolar biochemical analysis and molecular manipulation

Mark A. Shannon, Bruce R. Flachsbart, Jamie M. Iannacone, Kachuen Wong, Donald M. Cannon, Keqing Fa, Jonathan V. Sweedler, Paul W. Bohn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A multiple layer biochemical micro-analytical system has been developed that allows several functions to be conducted on mass limited (attomolar) samples of analytes that are in high backgrounds of constituent molecules. The functions are enabled by nanofluiclic interconnects that join separate microfluidic channels, which carry various buffer solutions, reactants, and products of different composition, concentration, and pH. The microfluidic channels are separated in the vertical direction by membranes with nanometer diameter cylindrical pores that serves as the nanofluidic interconnect. The functions this multilayer, micro-nanofluidic chip can perform include: (1) standard electrophoretic separations; (2) injections of attoliters of solution from one microfluidic channel into another with near instantaneous mixing and reaction; (3) collection and manipulation of specific analyte bands; (4) molecular size/mass dependent selective gating; (5) rapid mixing and chemical reactions between separate microfluidic channels; and (6) controlled maintenance of specie concentration, salt, buffer, and pH gradients between microfluidic analysis channels.

Original languageEnglish (US)
Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages257-259
Number of pages3
DOIs
StatePublished - Dec 1 2005
Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
Duration: May 12 2005May 15 2005

Publication series

Name2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Volume2005

Other

Other2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
CountryUnited States
CityOahu, HI
Period5/12/055/15/05

Keywords

  • Microfluidic analysis
  • Molecular gates
  • Nanofluidics

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Shannon, M. A., Flachsbart, B. R., Iannacone, J. M., Wong, K., Cannon, D. M., Fa, K., Sweedler, J. V., & Bohn, P. W. (2005). Nanofluidic interconnects within a multilayer microfluidic chip for attomolar biochemical analysis and molecular manipulation. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology (pp. 257-259). [1548442] (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology; Vol. 2005). https://doi.org/10.1109/MMB.2005.1548442