Nanoelectromechanics of methylated DNA in a synthetic nanopore

U. Mirsaidov, W. Timp, X. Zou, V. Dimitrov, K. Schulten, A. P. Feinberg, G. Timp

Research output: Contribution to journalArticlepeer-review


Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.

Original languageEnglish (US)
Pages (from-to)L32-L34
JournalBiophysical journal
Issue number4
StatePublished - Feb 18 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics


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