Nanodiscs in Membrane Biochemistry and Biophysics

Ilia G. Denisov, Stephen G. Sligar

Research output: Contribution to journalReview articlepeer-review

Abstract

Membrane proteins play a most important part in metabolism, signaling, cell motility, transport, development, and many other biochemical and biophysical processes which constitute fundamentals of life on the molecular level. Detailed understanding of these processes is necessary for the progress of life sciences and biomedical applications. Nanodiscs provide a new and powerful tool for a broad spectrum of biochemical and biophysical studies of membrane proteins and are commonly acknowledged as an optimal membrane mimetic system that provides control over size, composition, and specific functional modifications on the nanometer scale. In this review we attempted to combine a comprehensive list of various applications of nanodisc technology with systematic analysis of the most attractive features of this system and advantages provided by nanodiscs for structural and mechanistic studies of membrane proteins.

Original languageEnglish (US)
Pages (from-to)4669-4713
Number of pages45
JournalChemical reviews
Volume117
Issue number6
DOIs
StatePublished - Mar 22 2017

ASJC Scopus subject areas

  • Chemistry(all)

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