Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The interactions between lipids and proteins are one of the most fundamental processes in living organisms, responsible for critical cellular events ranging from replication, cell division, signaling, and movement. Enabling the central coupling responsible for maintaining the functionality of the breadth of proteins, receptors, and enzymes that find their natural home in biological membranes, the fundamental mechanisms of recognition of protein for lipid, and vice versa, have been a focal point of biochemical and biophysical investigations for many decades. Complexes of lipids and proteins, such as the various lipoprotein factions, play central roles in the trafficking of important proteins, small molecules and metabolites and are often implicated in disease states. Recently an engineered lipoprotein particle, termed the nanodisc, a modified form of the human high density lipoprotein fraction, has served as a membrane mimetic for the investigation of membrane proteins and studies of lipid–protein interactions. In this review, we summarize the current knowledge regarding this self-assembling lipid–protein complex and provide examples for its utility in the investigation of a large number of biological systems.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages645-671
Number of pages27
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2003
ISSN (Print)1064-3745

Keywords

  • Cytochrome P450
  • GPCR
  • Lipid–protein interactions
  • Lipoproteins
  • Membrane protein solubilization
  • Nanodiscs
  • Tissue factor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Denisov, I. G., Schuler, M. A., & Sligar, S. G. (2019). Nanodiscs as a New Tool to Examine Lipid–Protein Interactions. In Methods in Molecular Biology (pp. 645-671). (Methods in Molecular Biology; Vol. 2003). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9512-7_25