N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways

Mingxing Ouyang, Shaoying Lu, Taejin Kim, Chin En Chen, Jihye Seong, Deborah E. Leckband, Fei Wang, Albert B. Reynolds, Martin A. Schwartz, Yingxiao Wang

Research output: Contribution to journalArticle

Abstract

The spatial distribution of molecular signals within cells is crucial for cellular functions. Here, as a model to study the polarized spatial distribution of molecular activities, we used cells on micropatterned strips of fibronectin with one end free and the other end contacting a neighbouring cell. Phosphoinositide 3-kinase and the small GTPase Rac display greater activity at the free end, whereas myosin II light chain and actin filaments are enriched near the intercellular junction. Phosphoinositide 3-kinase and Rac polarization depend specifically on the N-cadherin-p120catenin complex, whereas myosin II light chain and actin filament polarization depend on the N-cadherin-β- catenin complex. Integrins promote high phosphoinositide 3-kinase/Rac activities at the free end, and the N-cadherin-p120catenin complex excludes integrin α5 at the junctions to suppress local phosphoinositide 3-kinase and Rac activity. We hence conclude that N-cadherin couples with distinct effectors to polarize phosphoinositide 3-kinase/Rac and myosin II light chain/actin filaments in migrating cells.

Original languageEnglish (US)
Article number1589
JournalNature communications
Volume4
DOIs
StatePublished - Apr 10 2013

Fingerprint

Catenins
1-Phosphatidylinositol 4-Kinase
Cadherins
Phosphatidylinositols
myosins
Myosin Type II
Phosphotransferases
Myosin Light Chains
filaments
Actin Cytoskeleton
Actins
cells
spatial distribution
Integrins
Spatial distribution
effectors
Polarization
polarization
Intercellular Junctions
Monomeric GTP-Binding Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways. / Ouyang, Mingxing; Lu, Shaoying; Kim, Taejin; Chen, Chin En; Seong, Jihye; Leckband, Deborah E.; Wang, Fei; Reynolds, Albert B.; Schwartz, Martin A.; Wang, Yingxiao.

In: Nature communications, Vol. 4, 1589, 10.04.2013.

Research output: Contribution to journalArticle

Ouyang, Mingxing ; Lu, Shaoying ; Kim, Taejin ; Chen, Chin En ; Seong, Jihye ; Leckband, Deborah E. ; Wang, Fei ; Reynolds, Albert B. ; Schwartz, Martin A. ; Wang, Yingxiao. / N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways. In: Nature communications. 2013 ; Vol. 4.
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AU - Leckband, Deborah E.

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