N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways

Mingxing Ouyang, Shaoying Lu, Taejin Kim, Chin En Chen, Jihye Seong, Deborah E. Leckband, Fei Wang, Albert B. Reynolds, Martin A. Schwartz, Yingxiao Wang

Research output: Contribution to journalArticle

Abstract

The spatial distribution of molecular signals within cells is crucial for cellular functions. Here, as a model to study the polarized spatial distribution of molecular activities, we used cells on micropatterned strips of fibronectin with one end free and the other end contacting a neighbouring cell. Phosphoinositide 3-kinase and the small GTPase Rac display greater activity at the free end, whereas myosin II light chain and actin filaments are enriched near the intercellular junction. Phosphoinositide 3-kinase and Rac polarization depend specifically on the N-cadherin-p120catenin complex, whereas myosin II light chain and actin filament polarization depend on the N-cadherin-β- catenin complex. Integrins promote high phosphoinositide 3-kinase/Rac activities at the free end, and the N-cadherin-p120catenin complex excludes integrin α5 at the junctions to suppress local phosphoinositide 3-kinase and Rac activity. We hence conclude that N-cadherin couples with distinct effectors to polarize phosphoinositide 3-kinase/Rac and myosin II light chain/actin filaments in migrating cells.

Original languageEnglish (US)
Article number1589
JournalNature communications
Volume4
DOIs
StatePublished - Apr 10 2013

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Ouyang, M., Lu, S., Kim, T., Chen, C. E., Seong, J., Leckband, D. E., Wang, F., Reynolds, A. B., Schwartz, M. A., & Wang, Y. (2013). N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways. Nature communications, 4, [1589]. https://doi.org/10.1038/ncomms2560