Myxoma virus expressing interleukin-15 fails to cause lethal myxomatosis in European rabbits

Jia Liu, Sonia Wennier, Mary Reinhard, Edward Roy, Amy MacNeill, Grant McFadden

Research output: Contribution to journalArticle

Abstract

Myxoma virus (MYXV) is a poxvirus pathogenic only for European rabbits, but its permissiveness in human cancer cells gives it potential as an oncolytic virus. A recombinant MYXV expressing both the tdTomato red fluorescent protein and interleukin-15 (IL-15) (vMyx-IL-15-tdTr) was constructed. Cells infected with vMyx-IL-15-tdTr secreted bioactive IL-15 and had in vitro replication kinetics similar to that of wild-type MYXV. To determine the safety of this virus for future oncolytic studies, we tested its pathogenesis in European rabbits. In vivo, vMyx-IL-15-tdTr no longer causes lethal myxomatosis. Thus, ectopic IL-15 functions as an antiviral cytokine in vivo, and vMyx-IL-15-tdTr is a safe candidate for animal studies of oncolytic virotherapy.

Original languageEnglish (US)
Pages (from-to)5933-5938
Number of pages6
JournalJournal of virology
Volume83
Issue number11
DOIs
StatePublished - Jun 1 2009

Fingerprint

Myxoma virus
myxomatosis
Interleukin-15
rabbits
Rabbits
Oncolytic Viruses
Oncolytic Virotherapy
Poxviridae
Permissiveness
viruses
interleukin-15
Antiviral Agents
cytokines
pathogenesis
Cytokines
Safety
kinetics

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Myxoma virus expressing interleukin-15 fails to cause lethal myxomatosis in European rabbits. / Liu, Jia; Wennier, Sonia; Reinhard, Mary; Roy, Edward; MacNeill, Amy; McFadden, Grant.

In: Journal of virology, Vol. 83, No. 11, 01.06.2009, p. 5933-5938.

Research output: Contribution to journalArticle

Liu, Jia ; Wennier, Sonia ; Reinhard, Mary ; Roy, Edward ; MacNeill, Amy ; McFadden, Grant. / Myxoma virus expressing interleukin-15 fails to cause lethal myxomatosis in European rabbits. In: Journal of virology. 2009 ; Vol. 83, No. 11. pp. 5933-5938.
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