Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization

Ahmet Yildiz, Joseph N. Forkey, Sean A. McKinney, Taekjip Ha, Yale E. Goldman, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ±37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of <1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 - 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.

Original languageEnglish (US)
Pages (from-to)2061-2065
Number of pages5
JournalScience
Volume300
Issue number5628
DOIs
StatePublished - Jun 27 2003

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Myosin Type V
Hand
Actins
Coloring Agents
Adenosine Triphosphate
Head
Observation
Light

ASJC Scopus subject areas

  • General

Cite this

Yildiz, A., Forkey, J. N., McKinney, S. A., Ha, T., Goldman, Y. E., & Selvin, P. R. (2003). Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization. Science, 300(5628), 2061-2065. https://doi.org/10.1126/science.1084398

Myosin V walks hand-over-hand : Single fluorophore imaging with 1.5-nm localization. / Yildiz, Ahmet; Forkey, Joseph N.; McKinney, Sean A.; Ha, Taekjip; Goldman, Yale E.; Selvin, Paul R.

In: Science, Vol. 300, No. 5628, 27.06.2003, p. 2061-2065.

Research output: Contribution to journalArticle

Yildiz, A, Forkey, JN, McKinney, SA, Ha, T, Goldman, YE & Selvin, PR 2003, 'Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization', Science, vol. 300, no. 5628, pp. 2061-2065. https://doi.org/10.1126/science.1084398
Yildiz, Ahmet ; Forkey, Joseph N. ; McKinney, Sean A. ; Ha, Taekjip ; Goldman, Yale E. ; Selvin, Paul R. / Myosin V walks hand-over-hand : Single fluorophore imaging with 1.5-nm localization. In: Science. 2003 ; Vol. 300, No. 5628. pp. 2061-2065.
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