Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue

Derek J. Milner; Massimo Bionaz; Elisa Monaco; Jo Ann Cameron; Matthew B. Wheeler

doi:10.1007/s00441-017-2764-z

Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue

Derek J. Milner, Massimo Bionaz, Elisa Monaco, Jo Ann Cameron, Matthew B. Wheeler

Research output: Contribution to journal › Article › peer-review

Abstract

Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C ₂ C ₁₂ myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C ₂ C ₁₂ cells resulted in GFP ⁺ myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP ⁺ ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.

Original language	English (US)
Pages (from-to)	507-522
Number of pages	16
Journal	Cell and Tissue Research
Volume	372
Issue number	3
DOIs	https://doi.org/10.1007/s00441-017-2764-z
State	Published - Jun 1 2018

Keywords

Adipose-derived stem cells
Muscle regeneration
Myogenesis
Porcine
Skeletal muscle

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology
Cell Biology

Online availability

10.1007/s00441-017-2764-z

Library availability

Discover UIUC Full Text

Cite this

@article{e75cea7b9019412899a204c45980c217,

title = "Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue",

abstract = " Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C 2 C 12 myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C 2 C 12 cells resulted in GFP + myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP + ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury. ",

keywords = "Adipose-derived stem cells, Muscle regeneration, Myogenesis, Porcine, Skeletal muscle",

author = "Milner, {Derek J.} and Massimo Bionaz and Elisa Monaco and Cameron, {Jo Ann} and Wheeler, {Matthew B.}",

note = "Funding Information: Acknowledgements The authors would like to thank Dr. Stephen Kaufman (University of Illinois) for his generous gift of polyclonal α7 integrin antibody, the NIH National Swine Resource and Research Center (NSRRC, University of Missouri-Columbia) for providing the GFP adipose tissue for the GFPASC isolation and Kathryn Polkoff (University of Illinois) for help with editing of the manuscript. A portion of the work presented here was partially supported by the Carle Foundation Hospital (#2007–04072), Urbana, IL and the Illinois Regenerative Medicine Institute (IDPH Grant # 63080017). Funding Information: The authors would like to thank Dr. Stephen Kaufman (University of Illinois) for his generous gift of polyclonal α7 integrin antibody, the NIH National Swine Resource and Research Center (NSRRC, University of Missouri-Columbia) for providing the GFP adipose tissue for the GFP ASC isolation and Kathryn Polkoff (University of Illinois) for help with editing of the manuscript. A portion of the work presented here was partially supported by the Carle Foundation Hospital (#2007–04072), Urbana, IL and the Illinois Regenerative Medicine Institute (IDPH Grant # 63080017). Publisher Copyright: {\textcopyright} 2018, Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2018",

month = jun,

day = "1",

doi = "10.1007/s00441-017-2764-z",

language = "English (US)",

volume = "372",

pages = "507--522",

journal = "Cell and Tissue Research",

issn = "0302-766X",

publisher = "Springer",

number = "3",

}

TY - JOUR

T1 - Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue

AU - Milner, Derek J.

AU - Bionaz, Massimo

AU - Monaco, Elisa

AU - Cameron, Jo Ann

AU - Wheeler, Matthew B.

N1 - Funding Information: Acknowledgements The authors would like to thank Dr. Stephen Kaufman (University of Illinois) for his generous gift of polyclonal α7 integrin antibody, the NIH National Swine Resource and Research Center (NSRRC, University of Missouri-Columbia) for providing the GFP adipose tissue for the GFPASC isolation and Kathryn Polkoff (University of Illinois) for help with editing of the manuscript. A portion of the work presented here was partially supported by the Carle Foundation Hospital (#2007–04072), Urbana, IL and the Illinois Regenerative Medicine Institute (IDPH Grant # 63080017). Funding Information: The authors would like to thank Dr. Stephen Kaufman (University of Illinois) for his generous gift of polyclonal α7 integrin antibody, the NIH National Swine Resource and Research Center (NSRRC, University of Missouri-Columbia) for providing the GFP adipose tissue for the GFP ASC isolation and Kathryn Polkoff (University of Illinois) for help with editing of the manuscript. A portion of the work presented here was partially supported by the Carle Foundation Hospital (#2007–04072), Urbana, IL and the Illinois Regenerative Medicine Institute (IDPH Grant # 63080017). Publisher Copyright: © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C 2 C 12 myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C 2 C 12 cells resulted in GFP + myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP + ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.

AB - Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C 2 C 12 myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C 2 C 12 cells resulted in GFP + myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP + ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.

KW - Adipose-derived stem cells

KW - Muscle regeneration

KW - Myogenesis

KW - Porcine

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=85040234068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040234068&partnerID=8YFLogxK

U2 - 10.1007/s00441-017-2764-z

DO - 10.1007/s00441-017-2764-z

M3 - Article

C2 - 29318389

AN - SCOPUS:85040234068

SN - 0302-766X

VL - 372

SP - 507

EP - 522

JO - Cell and Tissue Research

JF - Cell and Tissue Research

IS - 3

ER -

Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this