Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue

Derek J. Milner, Massimo Bionaz, Elisa Monaco, Jo Ann Cameron, Matthew B. Wheeler

Research output: Contribution to journalArticlepeer-review


Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C 2 C 12 myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C 2 C 12 cells resulted in GFP + myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP + ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.

Original languageEnglish (US)
Pages (from-to)507-522
Number of pages16
JournalCell and Tissue Research
Issue number3
StatePublished - Jun 1 2018


  • Adipose-derived stem cells
  • Muscle regeneration
  • Myogenesis
  • Porcine
  • Skeletal muscle

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


Dive into the research topics of 'Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue'. Together they form a unique fingerprint.

Cite this