Myocyte differentiation and body wall muscle regeneration in the planarian Girardia tigrina

Francesc Cebrià, Marcelo Vispo, Phillip Newmark, David Bueno, Rafael Romero

Research output: Contribution to journalArticlepeer-review


Freshwater planarians (Platyhelminthes, Turbellaria) show a great degree of morphological plasticity, making them a useful model for studying cell differentiation and pattern restoration processes during regeneration. Using confocal microscopy and a monoclonal antibody specific for muscle cells (TMUS-13), we have monitored the restoration of the body wall musculature during head regeneration in whole-mount organisms. Our results show that until the 4th day of regeneration the blastema is occupied by very disorganized muscle fibers, that from this moment become progressively organized restoring the original muscle pattern. In addition to recognizing mature muscle cells, TMUS-13 also recognizes differentiating myocytes, allowing us to trace the origin of newly formed muscle cells. We report that myocytes are detected in the postblastema region as early as day 1 of regeneration. This is the first demonstration that, in addition to serving as a proliferative zone as previously described, overt differentiation begins in the postblastema, at least for muscle cells. We also show that the TMUS13 antigen is the myosin heavy-chain gene from planarians.

Original languageEnglish (US)
Pages (from-to)306-316
Number of pages11
JournalDevelopment Genes and Evolution
Issue number5
StatePublished - 1997
Externally publishedYes


  • Muscle system
  • Myocyte differentiation
  • Myosin heavy chain
  • Planarian
  • Regeneration

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology


Dive into the research topics of 'Myocyte differentiation and body wall muscle regeneration in the planarian Girardia tigrina'. Together they form a unique fingerprint.

Cite this