Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins

Yonghua Hao, Zhizhou Kuang, Jia Jing, Jinfeng Miao, Li Yu Mei, Ryan J. Lee, Susie Kim, Shawn Choe, Duncan C. Krause, Gee Lau

Research output: Contribution to journalArticle

Abstract

Aberrant mucin secretion and accumulation in the airway lumen are clinical hallmarks associated with various lung diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis. Mycoplasma pneumoniae, long appreciated as one of the triggers of acute exacerbations of chronic pulmonary diseases, has recently been reported to promote excessive mucus secretion. However, the mechanism of mucin overproduction induced by M. pneumoniae remains unclear. This study aimed to determine the mechanism by which M. pneumoniae induces mucus hypersecretion by using M. pneumoniae infection of mouse lungs, human primary bronchial epithelial (NHBE) cells cultured at the air-liquid interface, and the conventionally cultured airway epithelial NCI-H292 cell line. We demonstrated that M. pneumoniae induced the expression of mucins MUC5AC and MUC5B by activating the STAT6-STAT3 and epidermal growth factor receptor (EGFR) signal pathways, which in turn downregulated FOXA2, a transcriptional repressor of mucin biosynthesis. The upstream stimuli of these pathways, including interleukin- 4 (IL-4), IL-6, and IL-13, increased dramatically upon exposure to M. pneumoniae. Inhibition of the STAT6, STAT3, and EGFR signaling pathways significantly restored the expression of FOXA2 and attenuated the expression of airway mucins MUC5AC and MUC5B. Collectively, these studies demonstrated that M. pneumoniae induces airway mucus hypersecretion by modulating the STAT/EGFR-FOXA2 signaling pathways.

Original languageEnglish (US)
Pages (from-to)5246-5255
Number of pages10
JournalInfection and immunity
Volume82
Issue number12
DOIs
StatePublished - Jan 1 2014

Fingerprint

Mycoplasma pneumoniae
Mucins
Epidermal Growth Factor Receptor
Mucus
Lung Diseases
Interleukin-13
Cystic Fibrosis
Interleukin-4
Chronic Obstructive Pulmonary Disease
Interleukin-6
Signal Transduction
Chronic Disease
Down-Regulation
Asthma
Epithelial Cells
Air
Cell Line
Lung
Infection

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases
  • Medicine(all)

Cite this

Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins. / Hao, Yonghua; Kuang, Zhizhou; Jing, Jia; Miao, Jinfeng; Mei, Li Yu; Lee, Ryan J.; Kim, Susie; Choe, Shawn; Krause, Duncan C.; Lau, Gee.

In: Infection and immunity, Vol. 82, No. 12, 01.01.2014, p. 5246-5255.

Research output: Contribution to journalArticle

Hao, Y, Kuang, Z, Jing, J, Miao, J, Mei, LY, Lee, RJ, Kim, S, Choe, S, Krause, DC & Lau, G 2014, 'Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins', Infection and immunity, vol. 82, no. 12, pp. 5246-5255. https://doi.org/10.1128/IAI.01989-14
Hao, Yonghua ; Kuang, Zhizhou ; Jing, Jia ; Miao, Jinfeng ; Mei, Li Yu ; Lee, Ryan J. ; Kim, Susie ; Choe, Shawn ; Krause, Duncan C. ; Lau, Gee. / Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins. In: Infection and immunity. 2014 ; Vol. 82, No. 12. pp. 5246-5255.
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