Mutations in an integration host factor-binding site

Effect on lambda site-specific recombination and regulatory implications

J. F. Thompson, D. Waechter-Brulla, R. I. Gumport, Jeffrey Gardner, L. Moitoso de Vargas, A. Landy

Research output: Contribution to journalArticle

Abstract

The manner in which integration host factor (IHF) regulates lambda site-specific recombination has been analyzed by examining the behavior of both wild-type and mutant DNAs in integrative and excisive recombination as well as in protein binding. While integrative recombination of an attP with two base changes in the H1 site required 8-fold more IHF than did wild type, binding to this site was lowered at least 500-fold, suggestive of cooperative interactions. A mutant attP with nine base changes did not integrate at all in vitro, with the defect being less severe in vivo. IHF inhibition of excisive recombination was relieved by both mutations in vitro and in vivo. These results imply that occupancy of the H1 site is critical for determining the direction of recombination. It is proposed that IHF inhibition of excision provides a monitor of the strength of the induction stimulus and the nutritional state of the cell; this would allow the prophage to exise selectivity in conditions which favor successful completion of the lytic cycle.

Original languageEnglish (US)
Pages (from-to)1343-1351
Number of pages9
JournalJournal of bacteriology
Volume168
Issue number3
DOIs
StatePublished - Jan 1 1986

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Integration Host Factors
Genetic Recombination
Binding Sites
Mutation
Prophages
Protein Binding
DNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Thompson, J. F., Waechter-Brulla, D., Gumport, R. I., Gardner, J., Moitoso de Vargas, L., & Landy, A. (1986). Mutations in an integration host factor-binding site: Effect on lambda site-specific recombination and regulatory implications. Journal of bacteriology, 168(3), 1343-1351. https://doi.org/10.1128/jb.168.3.1343-1351.1986

Mutations in an integration host factor-binding site : Effect on lambda site-specific recombination and regulatory implications. / Thompson, J. F.; Waechter-Brulla, D.; Gumport, R. I.; Gardner, Jeffrey; Moitoso de Vargas, L.; Landy, A.

In: Journal of bacteriology, Vol. 168, No. 3, 01.01.1986, p. 1343-1351.

Research output: Contribution to journalArticle

Thompson, JF, Waechter-Brulla, D, Gumport, RI, Gardner, J, Moitoso de Vargas, L & Landy, A 1986, 'Mutations in an integration host factor-binding site: Effect on lambda site-specific recombination and regulatory implications', Journal of bacteriology, vol. 168, no. 3, pp. 1343-1351. https://doi.org/10.1128/jb.168.3.1343-1351.1986
Thompson, J. F. ; Waechter-Brulla, D. ; Gumport, R. I. ; Gardner, Jeffrey ; Moitoso de Vargas, L. ; Landy, A. / Mutations in an integration host factor-binding site : Effect on lambda site-specific recombination and regulatory implications. In: Journal of bacteriology. 1986 ; Vol. 168, No. 3. pp. 1343-1351.
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